Subgroup C avian metapneumovirus (MPV) and the recently isolated human MPV exhibit a common organization but have extensive sequence divergence in their putative SH and G genes
| Autor: | Dennis A. Senne, Nicolas Eterradossi, Véronique Béven, D. Toquin, C. de Boisseson |
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| Rok vydání: | 2003 |
| Předmět: |
Genes
Viral G protein Molecular Sequence Data Retroviridae Proteins Oncogenic Biology Birds Viral Envelope Proteins Virology Animals Humans Metapneumovirus Amino Acid Sequence ORFS Gene Phylogeny Genetics chemistry.chemical_classification HN Protein Paramyxoviridae Infections Phylogenetic tree Bird Diseases Genetic Variation DNA-Directed RNA Polymerases Sequence Analysis DNA Avian pneumovirus Transmembrane domain chemistry Glycoprotein |
| Zdroj: | Journal of General Virology. 84:2169-2178 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.19043-0 |
| Popis: | The genes encoding the putative small hydrophobic (SH), attachment (G) and polymerase (L) proteins of the Colorado isolate of subgroup C avian pneumovirus (APV) were entirely or partially sequenced. They all included metapneumovirus (MPV)-like gene start and gene end sequences. The deduced Colorado SH protein shared 26·9 and 21·7 % aa identity with its counterpart in human MPV (hMPV) and APV subgroup A, respectively, but its only significant aa similarities were to hMPV. Conserved features included a common hydrophobicity profile with an unique transmembrane domain and the conservation of most extracellular cysteine residues. The Colorado putative G gene encoded several ORFs, the longer of which encoded a 252 aa long type II glycoprotein with aa similarities to hMPV G only (20·6 % overall aa identity with seven conserved N-terminal residues). The putative Colorado G protein shared, at best, 21·0 % aa identity with its counterparts in the other APV subgroups and did not contain the extracellular cysteine residues and short aa stretch highly conserved in other APVs. The N-terminal end of the Colorado L protein exhibited 73·6 and 54·9 % aa identity with hMPV and APV subgroup A, respectively, with four aa blocks highly conserved among Pneumovirinae. Phylogenetic analysis performed on the nt sequences confirmed that the L sequences from MPVs were genetically related, whereas analysis of the G sequences revealed that among MPVs, only APV subgroups A, B and D clustered together, independently of both the Colorado isolate and hMPV, which shared weak genetic relatedness at the G gene level. |
| Databáze: | OpenAIRE |
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