Pathogen‐inducible Ta‐Lr34res expression in heterologous barley confers disease resistance without negative pleiotropic effects
| Autor: | Anne C. Roulin, Jochen Kumlehn, Rainer Boni, Justine Sucher, Susanne Brunner, Harsh Chauhan, Beat Keller, Goetz Hensel, Simon G. Krattinger |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transgene Rust (fungus) Plant Science Plant disease resistance barley leaf rust 01 natural sciences 03 medical and health sciences Ascomycota Barley Cultivar Plant breeding Gene Research Articles Disease Resistance Plant Diseases 2. Zero hunger biology food and beverages Hordeum biology.organism_classification Plants Genetically Modified Horticulture Transformation (genetics) 030104 developmental biology Agronomy 13. Climate action durable disease resistance powdery mildew fungal pathogens Lr34/Yr18/Sr57/Pm38 Agronomy and Crop Science Powdery mildew 010606 plant biology & botany Biotechnology Research Article |
| Zdroj: | Plant Biotechnology Journal |
| ISSN: | 1467-7652 1467-7644 |
| Popis: | Summary Plant diseases are a serious threat to crop production. The informed use of naturally occurring disease resistance in plant breeding can greatly contribute to sustainably reduce yield losses caused by plant pathogens. The Ta‐Lr34res gene encodes an ABC transporter protein and confers partial, durable, and broad spectrum resistance against several fungal pathogens in wheat. Transgenic barley lines expressing Ta‐Lr34res showed enhanced resistance against powdery mildew and leaf rust of barley. While Ta‐Lr34res is only active at adult stage in wheat, Ta‐Lr34res was found to be highly expressed already at the seedling stage in transgenic barley resulting in severe negative effects on growth. Here, we expressed Ta‐Lr34res under the control of the pathogen‐inducible Hv‐Ger4c promoter in barley. Sixteen independent barley transformants showed strong resistance against leaf rust and powdery mildew. Infection assays and growth parameter measurements were performed under standard glasshouse and near‐field conditions using a convertible glasshouse. Two Hv‐Ger4c::Ta‐Lr34res transgenic events were analysed in detail. Plants of one transformation event had similar grain production compared to wild‐type under glasshouse and near‐field conditions. Our results showed that negative effects caused by constitutive high expression of Ta‐Lr34res driven by the endogenous wheat promoter in barley can be eliminated by inducible expression without compromising disease resistance. These data demonstrate that Ta‐Lr34res is agronomically useful in barley. We conclude that the generation of a large number of transformants in different barley cultivars followed by early field testing will allow identifying barley lines suitable for breeding. |
| Databáze: | OpenAIRE |
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