Preferential distribution of non-esterified fatty acids to phosphatidylcholine in the neonatal mammalian myocardium
| Autor: | C R Hartzell, N A Schroedl |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
Intracellular Fluid
Palmitic Acid Endogeny Oleic Acids Palmitic Acids Biology Fatty Acids Nonesterified Biochemistry chemistry.chemical_compound Biosynthesis Phosphatidylcholine Animals Molecular Biology Cells Cultured Growth medium Myocardium Cell Biology Metabolism Rats chemistry Animals Newborn Cell culture Phosphatidylcholines Chromatography Thin Layer Energy source Sphingomyelin Research Article Oleic Acid |
| Popis: | Non-esterified fatty acids are used to a limited extent as an energy source in the newborn-mammalian heart. Therefore additional roles for palmitic and oleic acids during this early period of growth and development were investigated in the cultured neonatal-rat heart cell model system. Our results indicate significant differences in nonesterified-fatty-acid metabolism exist in this system in comparison with the adult rat or embryonic chick heart. Initial rates of depletion of palmitate and oleate from serum-free growth medium by heart cells obtained from 2-day-old rats and maintained in culture for 10 or 11 days were 111 +/- 2 and 115 +/- 3 pmol/min per mg of protein respectively. In serum-containing medium, the initial depletion rates were 103 +/- 3 and 122 +/- 4 pmol/min per mg of protein respectively, when endogenous serum nonesterified-fatty-acid concentrations were included in rate calculations. Less than 1% of the intracellularly incorporated fatty acids were found in aqueous products at any time. After 25 h, 15.5% of the initial palmitate was deposited intracellularly in the phosphatidylcholine lipid fraction, 4.2% in the triacylglycerol + fatty-acid-ester fraction and 3.1% in the sphingomyelin fraction. These results contradict the classical view, based on findings with the lipid-dependent adult heart, that exogenous nonesterified fatty acids are directed intracellularly primarily to pathways of oxidation or to storage as triacylglycerol. More importantly, it underscores the significance of exogenous non-esterified fatty acids in membrane biosynthesis of the developing mammalian heart. Included here is a new method for one-dimensional t.l.c. separation of metabolically important polar lipids. |
| Databáze: | OpenAIRE |
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