Osteoblast-Based Therapy-A New Approach for Bone Repair in Osteoporosis: Pre-Clinical Setting
| Autor: | Hadeer A. Aglan, Mohamed A. M. Ali, Khalda Amr, Nadia S. Mahmoud, Mohamed R. Mohamed, Hanaa H. Ahmed |
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| Rok vydání: | 2020 |
| Předmět: |
Bone sialoprotein
Bone remodeling period musculoskeletal diseases Male medicine.medical_specialty 0206 medical engineering Osteoporosis Cathepsin K Biomedical Engineering Medicine (miscellaneous) Gene Expression 02 engineering and technology Bone healing Bone tissue Metabolic bone disease 03 medical and health sciences Osteogenesis Internal medicine Medicine Animals Femur 030304 developmental biology 0303 health sciences Osteoblasts biology business.industry RANK Ligand Osteoprotegerin Osteoblast Mesenchymal Stem Cells medicine.disease Alkaline Phosphatase 020601 biomedical engineering Rats Endocrinology medicine.anatomical_structure Durapatite RANKL biology.protein Original Article Bone Remodeling business |
| Zdroj: | Tissue Eng Regen Med |
| ISSN: | 2212-5469 |
| Popis: | BACKGROUND: Osteoporosis is a metabolic bone disease characterized by low bone density resulting in increased fracture susceptibility. This research was constructed to uncover the potential therapeutic application of osteoblasts transplantation, generated upon culturing male rat bone marrow-derived mesenchymal stem cells (BM-MSCs) in osteogenic medium (OM), OM containing gold (Au-NPs) or gold/hydroxyapatite (Au/HA-NPs) nanoparticles, in ovariectomized rats to counteract osteoporosis. METHODS: Forty rats were randomized into: (1) negative control, (2) osteoporotic rats, whereas groups (3), (4) and (5) constituted osteoporotic rats treated with osteoblasts yielded from culturing BM-MSCs in OM, OM plus Au-NPs or Au/HA-NPs, respectively. After 3 months, osterix (OSX), bone alkaline phosphatase (BALP), sclerostin (SOST) and bone sialoprotein (BSP) serum levels were assessed. In addition, gene expression levels of cathepsin K, receptor activator of nuclear factor-κb ligand (RANKL), osteoprotegerin (OPG) and RANKL/OPG ratio were evaluated using real-time PCR. Moreover, histological investigation of femur bone tissues in different groups was performed. The homing of implanted osteoblasts to the osteoporotic femur bone of rats was documented by Sex determining region Y gene detection in bone tissue. RESULTS: Our results indicated that osteoblasts infusion significantly blunted serum BALP, BSP and SOST levels, while significantly elevated OSX level. Also, they brought about significant down-regulation in gene expression levels of cathepsin K, RANKL and RANKL/OPG ratio versus untreated osteoporotic rats. Additionally, osteoblasts nidation could restore bone histoarchitecture. CONCLUSION: These findings offer scientific evidence that transplanting osteoblasts in osteoporotic rats regains the homeostasis of the bone remodeling cycle, thus providing a promising treatment strategy for primary osteoporosis. |
| Databáze: | OpenAIRE |
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