Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp
| Autor: | Jean Luiz Simões de Araújo, Luc Felicianus Marie Rouws, Jerri Édson Zilli, Marcia Maria Parma, Jakeline Renata Marçon Delamuta, Mariangela Hungria, Renan Augusto Ribeiro, Itamar Soares de Melo |
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| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Bradyrhizobium stylosanthis Sequence analysis 030106 microbiology medicine.disease_cause Microbiology Bradyrhizobium 03 medical and health sciences Intergenic region Genus Nitrogen Fixation RNA Ribosomal 16S DNA Ribosomal Spacer Botany medicine Phylogeny Ecology Evolution Behavior and Systematics Base Composition biology Fatty Acids Nucleic Acid Hybridization Fabaceae Sequence Analysis DNA General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification rpoB Bacterial Typing Techniques Housekeeping gene Genes Bacterial Stylosanthes bacteria Root Nodules Plant Multilocus Sequence Typing |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 66:3078-3087 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.001148 |
| Popis: | The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446T, BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense , and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4 % similarity to all described species of the genus Bradyrhizobium . Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with |
| Databáze: | OpenAIRE |
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