Functional polymorphism in lycopene beta-cyclase gene as a molecular marker to predict bixin production in Bixa orellana L. (achiote)
| Autor: | José Abraham Trujillo-Hdz, Pedro Escobar Turriza, Luz Elena Garza-Caligaris, Victor Manuel Carballo-Uicab, Renata Rivera-Madrid, Margarita Aguilar-Espinosa, Luca Comai, Yair Cárdenas-Conejo |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Crop and Pasture Production Heterosis Plant Biology & Botany SNP Plant Biology Outcrossing Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Molecular marker Botany Genetics Bo beta-LCY1 gene Genetic variability Genetic Testing Allele Molecular Biology Haplotype Human Genome Bixin Phenotypic trait Carotenoids SSCP 030104 developmental biology chemistry Agronomy and Crop Science Annatto 010606 plant biology & botany Biotechnology |
| Zdroj: | Molecular Breeding, vol 36, iss 9 Trujillo-Hdz, JA; Cárdenas-Conejo, Y; Turriza, PE; Aguilar-Espinosa, M; Carballo-Uicab, V; Garza-Caligaris, LE; et al.(2016). Functional polymorphism in lycopene beta-cyclase gene as a molecular marker to predict bixin production in Bixa orellana L. (achiote). Molecular Breeding, 36(9). doi: 10.1007/s11032-016-0555-y. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/0mh6r7kx |
| DOI: | 10.1007/s11032-016-0555-y. |
| Popis: | © 2016, Springer Science+Business Media Dordrecht. Bixin is an apocarotenoid obtained from the seed aril of Bixa orellana L., a tropical plant known as achiote in Mexico. This compound is the second most commonly used natural colouring for food and pharmaceutical industries. B. orellana is an outcrossing species that displays high genetic variability. Recently, the colour traits of sexual organs were associated with the biosynthesis and accumulation of bixin in mature seeds. Herein, we describe a new approach for genotype–phenotype association by surveying lycopene beta-cyclase (Boβ-LCY1) gene variation in sixteen achiote accessions divided into three groups according to contrasting traits, such as flower colour, fruit colour and bixin production. Using a combination of single-strand conformational polymorphism techniques and the sequencing of polymorphic bands, we identified several single-nucleotide polymorphisms that divided the accessions into three haplotypes. Surprisingly, we observed that these three haplotypes were consistent with the same three groups previously characterized by phenotypic traits. We derived a putative sequence for the Boβ-LCY1 gene and surveyed the variations in this sequence. The heterozygosity of Boβ-LCY1 alleles resulted in a higher bixin content, likely associated with heterosis for this metabolite. These findings augment the toolbox available for the selection and genetic improvement of B. orellana and provide a reliable phenotype–genotype association method for commercial varietal selection, contributing to the development of laboratory techniques to identify desirable traits of commercial plant species. |
| Databáze: | OpenAIRE |
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