Biomechanics as driver of aggregation of tethers in adherent membranes
| Autor: | Ana-Sunčana Smith, Kheya Sengupta, Franck Thibaudau, Mohammad Arif Kamal, Bernd Henning Stumpf, Long Li |
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| Přispěvatelé: | Centre Interdisciplinaire de Nanoscience de Marseille (CINaM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), ANR-18-CE92-0033,microCJ,Rôle du micro-environnement mécanique dans la formation précoce des jonctions cadhérines(2018) |
| Rok vydání: | 2021 |
| Předmět: |
Lipid Bilayers
Biophysics 02 engineering and technology Cell membrane 03 medical and health sciences chemistry.chemical_compound medicine [PHYS.COND]Physics [physics]/Condensed Matter [cond-mat] Cell adhesion Lipid bilayer Unilamellar Liposomes 030304 developmental biology [PHYS]Physics [physics] 0303 health sciences Membranes Physics Vesicle General Chemistry Adhesion 021001 nanoscience & nanotechnology Condensed Matter Physics Biomechanical Phenomena medicine.anatomical_structure Membrane chemistry 0210 nano-technology Linker DNA |
| Zdroj: | Soft Matter Soft Matter, 2021, 17 (44), pp.10101-10107. ⟨10.1039/d1sm00921d⟩ Soft Matter, Royal Society of Chemistry, 2021, 17 (44), pp.10101-10107. ⟨10.1039/D1SM00921D⟩ Soft Matter, 2021, 17 (44), pp.10101-10107. ⟨10.1039/D1SM00921D⟩ |
| ISSN: | 1744-6848 1744-683X |
| DOI: | 10.1039/d1sm00921d |
| Popis: | Cell adhesion is an important cellular process and is mediated by adhesion proteins residing on the cell membrane. Sometimes, two types of linker proteins are involved in adhesion, and they can segregate to form domains through a poorly understood size-exclusion process. We present an experimental and theoretical study of adhesion via linkers of two different sizes, realised in a mimetic model-system, based on giant unilamellar vesicles interacting with supported lipid bilayers. Here, adhesion is mediated by DNA linkers with two different lengths, but with the same binding enthalpy. We study the organisation of these linkers into domains as a function of relative fraction of long and short DNA constructs. Experimentally, we find that, irrespective of the composition, the adhesion domains are uniform with coexisting DNA bridge types, despite their relative difference in length of 9 nm. However, simulations suggest formation of nanodomains of the minority fraction at short length scales, which is below the optical resolution of the microscope. The nano-aggregation is more significant for long bridges, which are also more stable. |
| Databáze: | OpenAIRE |
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