All-Trans Retinoic Acid Shifts Propionibacterium acnes-Induced Matrix Degradation Expression Profile toward Matrix Preservation in Human Monocytes
Autor: | H. Ray Jalian, Philip T. Liu, Jenny Kim, Annaliza J. Legaspi, Jenny Phan, Melissa Kanchanapoomi |
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Jazyk: | angličtina |
Předmět: |
Chemokine
medicine.drug_class Retinoic acid Tretinoin Dermatology Matrix metalloproteinase Models Biological Biochemistry Gene Expression Regulation Enzymologic Monocytes Pathogenesis 030207 dermatology & venereal diseases 03 medical and health sciences Propionibacterium acnes chemistry.chemical_compound 0302 clinical medicine medicine Humans RNA Messenger Retinoid Molecular Biology 030304 developmental biology Tissue Inhibitor of Metalloproteinase-2 0303 health sciences Tissue Inhibitor of Metalloproteinase-1 biology Monocyte Toll-Like Receptors Gene Expression Regulation Bacterial Cell Biology biology.organism_classification Phenotype medicine.anatomical_structure chemistry Immune System Immunology biology.protein Cancer research medicine.drug |
Zdroj: | Journal of Investigative Dermatology. (12):2777-2782 |
ISSN: | 0022-202X |
DOI: | 10.1038/jid.2008.155 |
Popis: | Propionibacterium acnes is a critical component in the pathogenesis of acne vulgaris, stimulating the production of various inflammatory mediators, such as cytokines and chemokines, important in the local inflammatory response found in acne. This study explored the role of P. acnes and its ability to induce matrix metalloproteinases (MMPs) in primary human monocytes and how this induction is regulated by retinoids. MMP-1- and MMP-9-expressing cells were present in perifollicular and dermal inflammatory infiltrates within acne lesions, suggesting their role in acne pathogenesis. In vitro, we found that P. acnes induced MMP-9 and MMP-1 mRNA, and the expression of MMP-9, but not of MMP-1, was found to be Toll-like receptor 2-dependent. P. acnes induced the mRNA expression of tissue inhibitors of metalloproteinase (TIMP)-1, the main regulator of MMP-9 and MMP-1. Treatment of monocytes with all-trans retinoic acid (ATRA) significantly decreased baseline MMP-9 expression. Furthermore, co-treatment of monocytes with ATRA and P. acnes inhibited MMP-9 and MMP-1 induction, while augmenting TIMP-1 expression. These data indicate that P. acnes-induced MMPs and TIMPs may be involved in acne pathogenesis and that retinoic acid modulates MMP and TIMP expression, shifting from a matrix-degradative phenotype to a matrix-preserving phenotype. |
Databáze: | OpenAIRE |
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