No evidence of parvovirus B19 in tissue samples from patients with polyarteritis nodosa and microscopic polyangiitis as assessed by the polymerase chain reaction
| Autor: | Sandra Regina Muchinechi Fernandes, S C B Costa, Zoraida Sachetto, P D Andrade, Eliane Maria Ingrid Amstalden, Roseneide A. Conde, Adil Muhib Samara |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Vasculitis Pathology medicine.medical_specialty Adolescent viruses Immunology Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology law.invention Rheumatology law hemic and lymphatic diseases Parvovirus B19 Human medicine Humans Immunology and Allergy Letters Polymerase chain reaction biology Parvovirus Polyarteritis nodosa business.industry virus diseases Middle Aged biology.organism_classification medicine.disease Virology Polyarteritis Nodosa Giant cell DNA Viral Female Microscopic polyangiitis business Nested polymerase chain reaction Systemic vasculitis |
| Zdroj: | Annals of the Rheumatic Diseases. 65:418-420 |
| ISSN: | 0003-4967 |
| Popis: | Parvovirus B19 (B19) has been implicated in the aetiopathogenesis of some cases of systemic vasculitis, mainly giant cell arteritis.1 A positive serological test for B19 has been reported in a few patients with polyarteritis nodosa (PAN).2–4 As far as we know no studies of B19 DNA in tissue samples of patients with PAN have been published, so we used a nested polymerase chain reaction (PCR) approach to screen for B19 DNA in fixed paraffin embedded tissue samples from patients with PAN and microscopic polyangiitis (MPA). The diagnosis was based on the Chapell-Hill Consensus Conference guidelines.5 Only tissue samples from patients with vasculitis were used for DNA preparations. DNA was extracted using a commercial kit (DNeasy tissue kit, Quiagen). A nested PCR for B19 was carried out using a previously published set … |
| Databáze: | OpenAIRE |
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