Mutant alleles associated to chloroquine and sulfadoxine-pyrimethanime resistance in Plasmodium falciparum of the Ecuador-Peru and Ecuador-Colombia borders
| Autor: | Sonia Gutierrez, Raúl Veloz-Perez, Nancy Arróspide, Gisely Hijar-Guerra, César Cabezas Sánchez, César Eduardo Diaz-Cortéz, Doménica de Mora |
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| Předmět: |
Sulfadoxine
medicine.medical_treatment Mutant Plasmodium falciparum lcsh:Medicine DHPS Drug resistance Cloroquina medicine.disease_cause Resistencia bacteriana a fármacos Sulfadoxina Chloroquine parasitic diseases medicine bacterial Allele Genetics Mutation lcsh:R5-920 biology lcsh:R Public Health Environmental and Occupational Health General Medicine biology.organism_classification Virology Pyrimethamine Pirimetamina lcsh:Medicine (General) medicine.drug |
| Zdroj: | Redalyc Revista Peruana de Medicina Experimental y Salud Pública, Vol 31, Iss 2, Pp 282-287 Revista Peruana de Medicina Experimental y Salud Publica, Volume: 31, Issue: 2, Pages: 282-287, Published: APR 2014 |
| Popis: | Se evaluó la frecuencia de mutaciones en los genes pfCRT y DHFR/DHPS del Plasmodium falciparum asociados a la resistencia a cloroquina y sulfadoxina-pirimetamina en 83 cepas provenientes de los distritos Esmeralda y Machala ubicados en las fronteras entre Ecuador-Perú y Ecuador-Colombia durante el año 2002. Se empleó la reacción en cadena de polimerasa (PCR) convencional y sus variantes. El gen pfCRT presentó más de 90% de muestras mutantes en Esmeralda y Machala. Para el gen DHFR, el 90% de las cepas fueron muestras mutantes en Esmeralda, tres fueron mutaciones dobles y una triple; en Machala se encontró 25% de formas mutantes simples y 75% de formas mixtas (formas silvestres/mutantes). En conclusión, la resistencia a cloroquina se ha fijado en las cepas portadoras de la mutación K76T pfCRT, mientras que la impronta genética a la resistencia a pirimetamina está en evolución, principalmente en el distrito de Esmeralda The frequency of mutations in pfCRT and DHFR/DHPS genes of Plasmodium falciparum associated with resistance to chloroquine and sulfadoxine-pyrimethamine was evaluated in 83 strains from the districts of Esmeralda and Machala, located on the borders of Ecuador-Peru and Ecuador-Colombia in 2002. Polymerase chain reaction (PCR), conventional and its variants, was used. Mutations in the pfCRT gene were found in more than 90% of the samples from Esmeralda and Machala. For the DHFR gene, 90% of the strains were mutant samples from Esmeralda, 3 were double mutations and 1 was a triple mutation. In Machala, 25% were simple mutant forms and 75% mixed mutant forms (wild forms/mutant). In conclusion, resistance to chloroquine has been fixed in strains carrying K76T pfCRT mutation, whereas genetic imprinting for resistance to pyrimethamine is evolving, particularly in the district of Esmeralda |
| Databáze: | OpenAIRE |
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