Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo
| Autor: | Jean-Rémi Bertrand, Claude Malvy, Andrei Maksimenko, Mireille Pottier, Paule Opolon, Antoine Vekris |
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| Rok vydání: | 2002 |
| Předmět: |
Small interfering RNA
RNA Untranslated Time Factors Green Fluorescent Proteins Biophysics Biochemistry Green fluorescent protein HeLa Mice In vivo Nucleic Acids Animals Humans RNA Messenger RNA Small Interfering Molecular Biology Cells Cultured Nuclease biology Oligonucleotide RNA Cell Biology Oligonucleotides Antisense Blotting Northern biology.organism_classification Immunohistochemistry Molecular biology Blot Luminescent Proteins Genetic Techniques biology.protein HeLa Cells |
| Zdroj: | Biochemical and Biophysical Research Communications. 296:1000-1004 |
| ISSN: | 0006-291X |
| Popis: | Efficiencies of a nuclease resistant antisense oligonucleotide and of siRNA both being targeted against the green fluorescent protein stably expressed in HeLa cells are compared in cell cultures and in xenografted mice. Using Cytofectin GSV to deliver both inhibitors, the siRNAs appear to be quantitatively more efficient and its effect is lasting for a longer time in cell culture. In mice, we observed an activity of siRNAs but not of antisense oligonucleotides. The absence of efficiency of antisense oligonucleotides is probably due to their lower resistance to nuclease degradation. |
| Databáze: | OpenAIRE |
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