Comprehensive Transcriptome Analyses in Sea Louse Reveal Novel Delousing Drug Responses Through MicroRNA regulation
| Autor: | Diego Valenzuela-Miranda, Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña |
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| Rok vydání: | 2021 |
| Předmět: |
Sea louse
Salmo salar Drug Resistance Drug resistance Applied Microbiology and Biotechnology Copepoda Transcriptome Fish Diseases chemistry.chemical_compound Pyrethrins Azamethiphos microRNA Animals Gene ADME Regulation of gene expression Genetics Antiparasitic Agents biology Gene Expression Profiling Organothiophosphates biology.organism_classification MicroRNAs Gene Expression Regulation chemistry RNA Long Noncoding |
| Zdroj: | Marine Biotechnology. 23:710-723 |
| ISSN: | 1436-2236 1436-2228 |
| DOI: | 10.1007/s10126-021-10058-z |
| Popis: | The role of miRNAs in pharmacological responses through gene regulation related to drug metabolism and the detoxification system has recently been determined for terrestrial species. However, studies on marine ectoparasites have scarcely been conducted to investigate the molecular mechanisms of pesticide resistance. Herein, we explored the sea louse Caligus rogercresseyi miRNome responses exposed to delousing drugs and the interplaying with coding/non-coding RNAs. Drug sensitivity in sea lice was tested by in vitro bioassays for the pesticides azamethiphos, deltamethrin, and cypermethrin. Ectoparasites strains with contrasting susceptibility to these compounds were used. Small-RNA sequencing was conducted, identifying 2776 novel annotated miRNAs, where 163 mature miRNAs were differentially expressed in response to the drug testing. Notably, putative binding sites for miRNAs were found in the ADME genes associated with the drugs' absorption, distribution, metabolism, and excretion. Interactions between the miRNAs and long non-coding RNAs (lncRNAs) were also found, suggesting putative molecular gene regulation mechanisms. This study reports putative miRNAs correlated to the coding/non-coding RNAs modulation, revealing novel pharmacological mechanisms associated with drug resistance in sea lice species. |
| Databáze: | OpenAIRE |
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