High‐risk human papilloma virus was not detected in a Norwegian cohort of oral squamous cell carcinoma of the mobile tongue

Autor: Inger-Heidi Bjerkli, Jeanette Bæhr Georgsen, Peter Jebsen, T.M. Søland, Olaf Joseph Franciscus Schreurs, Helene Laurvik, Dipak Sapkota
Rok vydání: 2020
Předmět:
squamous cell carcinoma
Cell
In situ hybridization
Tongue
human papilloma virus
Biomarkers
Tumor
medicine
Humans
cancer of head and neck
cancer of the mouth
General Dentistry
Tissue microarray
VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762
Squamous Cell Carcinoma of Head and Neck
business.industry
Papillomavirus Infections
Original Articles
VDP::Medisinske Fag: 700::Klinisk odontologiske fag: 830
VDP::Medical disciplines: 700::Clinical dentistry disciplines: 830
Tongue Neoplasms
Staining
lcsh:RK1-715
VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762
stomatognathic diseases
medicine.anatomical_structure
Head and Neck Neoplasms
lcsh:Dentistry
immunohistochemistry
Cancer cell
Cohort
Carcinoma
Squamous Cell
Cancer research
Immunohistochemistry
Original Article
Mouth Neoplasms
in situ hybridization
business
Zdroj: Clinical and Experimental Dental Research
Clinical and Experimental Dental Research, Vol 7, Iss 1, Pp 70-77 (2021)
Søland, T M, Bjerkli, I H, Georgsen, J B, Schreurs, O, Jebsen, P, Laurvik, H & Sapkota, D 2021, ' High-risk human papilloma virus was not detected in a Norwegian cohort of oral squamous cell carcinoma of the mobile tongue ', Clinical and Experimental Dental Research, vol. 7, no. 1, pp. 70-77 . https://doi.org/10.1002/cre2.342
ISSN: 2057-4347
DOI: 10.1002/cre2.342
Popis: Objectives - The presence of and the causative role of high‐risk human papilloma virus (HPV) is a subject of controversy in oral squamous cell carcinoma (OSCC). The disagreement can be related to the misclassification of OSCC as oropharyngeal squamous cell carcinoma and/or lack of standard detection methods. This study aimed to examine the presence of transcriptionally active high‐risk HPV in a homogenous Norwegian cohort of primary and second primary OSCC of the mobile tongue (oral tongue squamous cell carcinoma—OTSCC). Methods - Tissue microarrays containing formalin‐fixed and paraffin‐embedded cores of 146 OTSCC from the anterior 2/3 of the tongue (n = 128 primary and n = 18 second primary) from a multicentric Norwegian cohort were examined for the presence of high‐risk HPV by DNA‐ and RNA‐in situ hybridization (ISH) assays and p16 immunohistochemistry. Results -Transcriptionally active HPV (E6/E7 mRNA) was not identified in any of the OTSCC specimens. In parallel, no tumors were positive for HPV by DNA ISH. Although, 61 (42%) OTSCC demonstrated p16 positivity with varying staining intensity and subcellular localization, only two cases demonstrated strong and uniform p16‐staining (both cytoplasmic and nuclear) in >70% of cancer cells. The absence of transcriptionally active high‐risk HPV in this cohort of OTSCC indicates that high‐risk HPV is an unlikely causative factor in the present material.
Databáze: OpenAIRE
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