Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows
| Autor: | P.J. Hartigan, William J. Meaney, Cyril J. Smyth, Vivek Kapur, J.R. Fitzgerald |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
clone (Java method)
DNA Bacterial Veterinary medicine Staphylococcus aureus Epidemiology Mastitis Bovine/microbiology Electrophoresis Starch Gel Restriction Mapping Ireland/epidemiology Biology medicine.disease_cause Sensitivity and Specificity Microbiology Mastitis Bovine/epidemiology Ribotyping Staphylococcal Infections/veterinary Genotype medicine Animals Typing Mastitis Bovine Staphylococcal Infections/epidemiology Molecular Epidemiology Molecular epidemiology Discriminant Analysis Staphylococcal Infections medicine.disease United States/epidemiology United States Mastitis RAPD Random Amplified Polymorphic DNA Technique Staphylococcal Infections/microbiology Staphylococcus aureus/genetics DNA Bacterial/genetics Infectious Diseases Cattle Female Ireland Research Article |
| Zdroj: | Fitzgerald, R, Meaney, W J, Hartigan, P J, Smyth, C J & Kapur, V 1997, ' Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows ', Epidemiology and Infection, vol. 119, no. 2, pp. 261-9 . |
| Popis: | Sixty-three Staphylococcus aureus isolates recovered from bovine sources in the USA and the Republic of Ireland were characterized by multilocus enzyme electrophoresis (MLEE), ribotyping, and random amplified polymorphic DNA polymerase chain reaction (RAPD–PCR) typing at two separate laboratories. The S. aureus isolates were assigned by MLEE to 10 electrophoretic types (ETs) (Index of Discrimination, D=0·779). In contrast, the same isolates were assigned to 13 ribotypes (D=0·888), and to 12 RAPD types (D=0·898). A common clone, ET3, of worldwide distribution, was represented by six distinct combinations of ribotypes and RAPD types. S. aureus clones recovered from cows in Ireland were also associated with mastitis in dairy cows in the USA. These findings are consistent with the hypothesis that only a few specialized clones of S. aureus are responsible for the majority of cases of bovine mastitis, and that these clones have a broad geographic distribution. |
| Databáze: | OpenAIRE |
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