Adenosine 5′ phosphosulfate reductase and sulfite oxidase regulate sulfite-induced water loss in Arabidopsis
Autor: | Zhadyrassyn Nurbekova, Moshe Sagi, Assylay Kurmanbayeva, Dinara Oshanova, Veronika Turečková, Sudhakar Srivastava, Poonam Tiwari, Miroslav Strnad, Aigerim Soltabayeva, Aizat Bekturova, Dmitry Yarmolinsky |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine animal structures Physiology Mutant Glutathione reductase Arabidopsis Plant Science Reductase 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Sulfite Sulfite oxidase Sulfites Oxidoreductases Acting on Sulfur Group Donors NADPH oxidase biology Arabidopsis Proteins Sulfite Oxidase Water Hydrogen Peroxide Glutathione Glutathione Reductase 030104 developmental biology Biochemistry chemistry biology.protein 010606 plant biology & botany Cysteine |
Zdroj: | Journal of Experimental Botany. 72:6447-6466 |
ISSN: | 1460-2431 0022-0957 |
Popis: | Chloroplast-localized adenosine-5'-phosphosulphate reductase (APR) generates sulfite and plays a pivotal role in sulfate reduction to cysteine. The peroxisome-localized sulfite oxidase (SO), oxidizes excess sulfite to sulfate. Wild-type (WT), SO RNA-interference (SO Ri) and SO overexpression (SO OE) Arabidopsis mutants were infiltrated with sulfite. In SO Ri plants, water loss was increased due to enhancement of stomatal aperture compared to WT leaves, whereas in SO OE plants, stomatal aperture was smaller than that of the WT plants, and hence water loss was lower. Sulfite application also limited sulfate and ABA-induced stomatal closure in WT and SO Ri. The increases in APR activity in response to sulfite infiltration into WT and SO Ri leaves resulted in an increase in sulfite beyond the level of the applied sulfite, indicating that APR has an important role in sulfite-induced increases in stomatal aperture. Notably, sulfite-induced H2O2 generation by NADPH oxidase, led to enhanced APR expression and sulfite production. Suppression of APR by inhibiting NADPH oxidase and glutathione reductase2 (GR2) by diphenyleneiodonium, or mutation in APR2 or GR2, resulted in decrease in sulfite production and stomatal aperture size, further supporting the role of APR in stomatal aperture size. The importance of APR and SO in the set-up of sulfite level in leaves, and the significance of sulfite level in water loss were further demonstrated during fast and harsh drought stress in root-detached WT, gr2 and SO modified plants. The role of SO in sulfite homeostasis in relation to water consumption was shown in well-watered plants. |
Databáze: | OpenAIRE |
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