Fast Determination of Ingredients in Solid Pharmaceuticals by Microwave-Enhanced In-Source Decay of Microwave Plasma Torch Mass Spectrometry
| Autor: | Rui Su, Huanwen Chen, Yang Meiling, Wang Xinchen, Keke Huang, Changming Hou |
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| Rok vydání: | 2016 |
| Předmět: |
Ambient ionization
Analytical chemistry 010402 general chemistry Mass spectrometry 01 natural sciences Sample preparation in mass spectrometry Structural Biology Limit of Detection Microwaves Inductively coupled plasma mass spectrometry Spectroscopy Microwave plasma torch Desorption electrospray ionization Chromatography Chemistry 010401 analytical chemistry Selected reaction monitoring Qualitative and quantitative analysis 0104 chemical sciences High-Throughput Screening Assays Models Chemical Pharmaceutical Preparations Drug screening Mass spectrum Mechanism Time-of-flight mass spectrometry Research Article |
| Zdroj: | Journal of the American Society for Mass Spectrometry |
| ISSN: | 1879-1123 |
| Popis: | Rapid qualitative and quantitative analysis of solid samples (e.g., pharmaceutical preparations) by using a small and low-resolution mass spectrometer without MS/MS function is still a challenge in ambient pressure ionization mass spectrometric analysis. Herein, a practically efficient method termed microwave-enhanced in-source decay (MEISD) using microwave plasma torch desorption ionization coupled with time-of-flight mass spectrometry (MPTDI-TOF MS) was developed for fast analysis of pharmaceutical tablets using a miniature TOF mass spectrometer without tandem mass function. The intensity of ISD fragmentation was evaluated under different microwave power values. Several factors, including desorption distance and time that might affect the signal intensity and fragmentation, were systematically investigated. It was observed that both the protonated molecular ions and major fragment ions from the active ingredients in tablets could be found in the full-scan mass spectra in positive ion mode, which were comparable to those obtained by a commercial LTQ-XL ion trap mass spectrometer. The structures of the ingredients could be elucidated in detail using the MEISD method, which promotes our understanding of the desorption/ionization processes in microwave plasma torch (MPT). Quantitative analysis of 10 tablets was achieved by full-scan MPTDI-TOF MS with low limit of detection (LOD, 0.763 mg/g), acceptable relative standard deviation (RSD < 7.33%, n =10), and 10 s for each tablet, showing promising applications in high throughput screening of counterfeit drugs. Graphical Abstractᅟ Electronic supplementary material The online version of this article (doi:10.1007/s13361-017-1708-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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