Chromosome instability as an indicator of malignant progression in laryngeal mucosa
| Autor: | Joris A. Veltman, Johannes J. Manni, Fredrik J. Bot, Anton H. N. Hopman, Frans C. S. Ramaekers, Ference C. Huynen |
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| Přispěvatelé: | Gynaecologie en Obstetrie, Pathologie, Moleculaire Celbiologie, Keel-, Neus- en Oorheelkunde, RS: GROW - School for Oncology and Reproduction |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Larynx
Cancer Research Pathology medicine.medical_specialty medicine.disease_cause Polyploidy Chromosome instability medicine Carcinoma Humans De rol van chromosoomafwijkingen en (anti-)oncogenen in humane tumoren Laryngeal Neoplasms In Situ Hybridization Chromosome Aberrations Hyperplasia business.industry Cytogenetics Cancer medicine.disease Prognosis medicine.anatomical_structure Oncology Tumor progression Chromosomes Human Pair 1 Laryngeal Mucosa Carcinoma Squamous Cell Disease Progression The role of chromosomal aberrations and (anti-)oncogenes in human tumours business Carcinogenesis Precancerous Conditions Carcinoma in Situ Chromosomes Human Pair 7 |
| Zdroj: | Journal of Clinical Oncology, 18, pp. 1644-1651 Journal of Clinical Oncology, 18, 1644-1651 Journal of Clinical Oncology, 18, 1644-51. American Society of Clinical Oncology |
| ISSN: | 1527-7755 0732-183X |
| Popis: | PURPOSE: Routine histologic examination cannot predict whether premalignant laryngeal lesions will progress toward invasive growth. The acquisition of changes in chromosome constitution has been suggested to be essential for driving tumor progression by enhancing mutagenic mechanisms. The aim of the present study was to determine whether chromosomal changes occur in the subsequent stages of early laryngeal carcinogenesis and, if so, whether these changes can be of prognostic value. MATERIALS AND METHODS: Numerical aberrations for chromosomes 1 and 7 were detected in tissue sections from archival material using an improved in situ hybridization protocol. In total, eight benign laryngeal lesions, 37 premalignant laryngeal lesions, and 16 specimens containing histologically normal epithelia adjacent to laryngeal squamous cell carcinomas were studied. Both the histologic and the cytogenetic classifications were correlated with progression to laryngeal cancer. RESULTS: No evidence for chromosome alterations was obtained in the control group, nor in histologically normal epithelia adjacent to laryngeal squamous cell carcinomas, nor in all but one hyperplastic lesion (n = 11). In contrast, 14 of 15 dysplastic lesions and nine of 11 carcinomas-in-situ contained numerical chromosomal aberrations. Tetrasomy was present in the majority of the dysplastic lesions. An unstable chromosome content (indicated by the presence of chromosome imbalances and/or polyploidization) in the premalignant lesion strongly predicted its malignant progression. CONCLUSION: Our results show that laryngeal tumor development involves chromosome tetraploidization. The further change from a stable to an unstable chromosome constitution is of importance for malignant progression. |
| Databáze: | OpenAIRE |
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