Genotoxicity evaluation of titanium dioxide nanoparticles using the mouse lymphoma assay and the Ames test
| Autor: | Jihong Wu, Xiuming Du, Shunxiang Gao, Qingyun Zhou, Xin Zheng, Liling Hong |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Salmonella typhimurium
0301 basic medicine Salmonella Health Toxicology and Mutagenesis Metal Nanoparticles 010501 environmental sciences Gene mutation medicine.disease_cause 01 natural sciences Ames test Mice 03 medical and health sciences Mutation Rate Cell Line Tumor Genetics medicine Animals Mutation frequency 0105 earth and related environmental sciences Titanium Mutagenicity Tests Chemistry Mouse Lymphoma technology industry and agriculture Molecular biology In vitro 030104 developmental biology Mutagenesis Titanium dioxide nanoparticles Genotoxicity DNA Damage |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 838:22-27 |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/j.mrgentox.2018.11.015 |
| Popis: | Titanium dioxide nanoparticles (TiO2-NPs) are widely used in the cosmetics, health, and food industries, but their safety and genotoxicity remain a matter of debate. We investigated whether TiO2-NPs could induce gene mutations in mouse lymphoma L5178Y cells and Salmonella typhimurium strains TA97a, TA98, TA100, TA102, and TA1535. Following preliminary tests, 2 mg/mL for the mouse lymphoma gene mutation assay and 1.25 mg/plate for the in vitro bacterial reverse mutation assay (Ames test) were selected as the highest concentrations. Exposure to TiO2-NPs for 4 or 24 h with or without S9 metabolic activation did not increase mutation frequency for any of the concentrations tested in L5178Y cells. In the Ames test, TiO2-NPs did not induce reverse mutation in the bacterial strains. No positive mutagenic responses were observed in either test system, and therefore we cannot classify TiO2-NPs as mutagenic; further testing will be required to determine conclusively whether TiO2-NPs are genotoxic. |
| Databáze: | OpenAIRE |
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