Detection of the 170 kDa P-Glycoprotein in Neoplastic and Normal Tissues
| Autor: | G. Di Fronzo, S. Catania, E. Ronchi, Rosella Silvestrini, O. Sanfilippo, G. Della Torre, M R Bani |
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| Rok vydání: | 1989 |
| Předmět: |
Cancer Research
Fibrosarcoma Blotting Western Drug Resistance 030218 nuclear medicine & medical imaging Mice 03 medical and health sciences 0302 clinical medicine Reference Values In vivo Microsomes Neoplasms Tumor Cells Cultured medicine Animals Humans ATP Binding Cassette Transporter Subfamily B Member 1 P-glycoprotein chemistry.chemical_classification Mice Inbred C3H Membrane Glycoproteins biology General Medicine medicine.disease Primary tumor Molecular biology In vitro Neoplasm Proteins Blot Oncology chemistry Membrane protein Doxorubicin 030220 oncology & carcinogenesis biology.protein Electrophoresis Polyacrylamide Gel Glycoprotein |
| Zdroj: | Tumori Journal. 75:542-546 |
| ISSN: | 2038-2529 0300-8916 |
| DOI: | 10.1177/030089168907500605 |
| Popis: | A membrane purification procedure and an immunoblotting assay have been designed to allow screening of human solid tumors for overexpression of the GP170 glycoprotein without employing a disaggregation method to obtain cell suspensions. The electrophoresed membrane proteins were probed, after Western Blotting, with the C219 monoclonal antibody and iodinated Protein A. The labeling intensity of the bands on the autoradioimmunoblots were quantified by densitometry. To test for the presence of GP170, we used membranes from the UV 2237 fibrosarcoma line and its adriamycin-resistant variant ADMR, grown in vitro or as solid tumor in mice. Membranes of human normal and tumor tissues obtained from previously untreated patients were also tested. An immunoreaction was observed in the adriamycin-resistant UV 2237 lines grown in vitro or in vivo. Quantitatively, the binding of the resistant cell line grown in vitro was higher than that observed in cells grown in mice. Bands in the GP 170 region were observed in 4/7 normal and in 7/7 tumor colon tissues and in the normal medulla from 2 patients with cancer of the renal cortex. No reaction could be found in samples from normal tissue, primary tumor or nodal metastasis from 7 patients with breast cancer. |
| Databáze: | OpenAIRE |
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