Immunohistochemical response in rats of beta-tricalcium phosphate (TCP) with or without BMP-2 in the production of collagen matrix critical defects
| Autor: | Pedro Henrique Silva Gomes-Ferreira, Roberta Okamoto, Leonardo Perez Faverani, Cristina Antoniali, Júlio César Silva de Oliveira, Cassiano Costa Silva Pereira, Eloá Rodrigues Luvizuto |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Calcium Phosphates
Male medicine.medical_specialty Histology Dentistry Bone Morphogenetic Protein 2 Calvaria 02 engineering and technology Bone healing Bone morphogenetic protein 2 Bone and Bones 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals BMP Rats Wistar Wound Healing biology business.industry Skull Biomaterial 030206 dentistry Cell Biology General Medicine 021001 nanoscience & nanotechnology Phosphate Immunohistochemistry Rats Histologic evaluation medicine.anatomical_structure Endocrinology chemistry Osteocalcin biology.protein Collagen matrix Collagen 0210 nano-technology business Wound healing |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-12-11T17:31:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-04-01 This study aimed to assess the biological response of BMP-2 (bone morphogenetic protein-2) in supplementation with β-tricalcium phosphate (TCP) as a carrier in the bone healing of surgical defects in rats’ calvaria. A critical-size defect (5 mm in diameter) was filled with β-TCP alone or added with that plus 5 mg of BMP-2 at 5, 15, and 30 postoperative days. Histomorphometric and immunohistochemical (osteocalcin, collagen type I, and metalloproteinase-9) analysis was performed to assess the features of bone healing. Histological behavior and collagen type I labeling showed increased formation of the collagen matrix, leading to a higher percentage of newly formed bone and biomaterial for tissue and more total mineralization of pure TCP when compared to the other groups. The supplementation with BMP-2 promoted faster TCP remodeling; however, there was no statistically significant difference for the bone formed in both groups (P > 0.05). Collagen-matrix formation and new bone formation reached maximum levels when the defects were filled with pure TCP, even exceeding the levels from BMP-2 supplementation. Department of Surgery and Integrated Clinic Sao Paulo State University (UNESP) School of Dentistry Department of Basic Sciences Sao Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic Sao Paulo State University (UNESP) School of Dentistry Department of Basic Sciences Sao Paulo State University (UNESP) School of Dentistry |
| Databáze: | OpenAIRE |
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