Changes in the cytokine profile of lupus-prone mice (NZB/NZW)F1 induced byPlasmodium chabaudiand their implications in the reversal of clinical symptoms
| Autor: | S Avrameas, Maria Notomi Sato, P Minoprio, T Ternynck |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Interleukin 2
T-Lymphocytes medicine.medical_treatment T cell Immunology Immunoglobulins Lymphocyte Activation Rheumatic Disease Plasmodium chabaudi Mice Concanavalin A medicine Animals Lupus Erythematosus Systemic Immunology and Allergy Genetic Predisposition to Disease RNA Messenger Interleukin 5 Crosses Genetic Interleukin 4 Lymphokines Mice Inbred BALB C Mice Inbred NZB biology biology.organism_classification Malaria Interleukin 10 Cytokine medicine.anatomical_structure Cytokines Female Cytokine secretion medicine.drug |
| Zdroj: | Publons |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.2000.01124.x |
| Popis: | SUMMARYWe have previously observed that aged lupus-prone (NZB/NZW)Fl (BWF1) mice when infected with Plasmodium chabaudi show an improvement in their clinical lupus-like symptoms. In order to study the mechanisms involved in the long-lasting protective effect of the P. chabaudi infection in lupus-prone mice we analysed specific aspects of the cellular response, namely the profiles of cytokine mRNA expression and cytokine secretion levels in old BWF1 mice, in comparison with uninfected age-matched BWF1 mice and infected or uninfected BALB/c mice. Two months after infection, cells from BWF1 mice were stimulated with concanavalin A (Con A) and demonstrated a recovery of T cell responsiveness that reached the levels obtained with BALB/c cells. Old BWF1 mice showed high levels of interferon-gamma (IFN-γ) and IL-5 production and correspondingly low levels of IL-2 and IL-4 secretion before infection with P. chabaudi. Infection did not modify the IFN-γ levels of BWF1 T cells, whereas it considerably increased the secretion of the Th2-related cytokines IL-4, IL-5 and IL-10. In addition, only BWF1 T cells showed increased mRNA expression of tumour necrosis factor-alpha (TNF-α) and transforming growth factor-beta (TGF-β). This counter-regulatory cytokine network of infected BWF1 mice may be involved in the improvement of their lupus symptoms. The results of our investigations using the complex model of P. chabaudi infection can be extended and, by using more restricted approaches, it may be possible to explain the multiple regulatory defects of lupus-prone mice. |
| Databáze: | OpenAIRE |
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