Development of mPing-based Activation Tags for Crop Insertional Mutagenesis
| Autor: | Thomas E. Clemente, Lauren A. L. McDaniel, C. Nathan Hancock, Edward V. McAssey, Wayne A. Parrott, Alexander Johnson, Hanh Nguyen, Robert M. Stupar, Jacob Reagin, Stephanie Diaz, Daymond R. Parrilla, Adrian O. Stec |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
2. Zero hunger 0303 health sciences Transgene Mutagenesis (molecular biology technique) food and beverages Computational biology Biology 01 natural sciences Phenotype Transposition (music) Insertional mutagenesis 03 medical and health sciences Gene Gene Discovery Transposase 030304 developmental biology 010606 plant biology & botany |
| DOI: | 10.1101/2020.04.22.055970 |
| Popis: | Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, themPingelement from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences inmPingresulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version ofmPingcalledmmPing20. Transgenic soybean events carryingmPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of themmPing20Factivation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the originalmPingelement, improving the overall effectiveness of the mutagenesis system. |
| Databáze: | OpenAIRE |
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