Isolation and partial characterization of an opioid-like 88 kDa hibernation-related protein
| Autor: | Mitchell S. Turker, Nada H. Khattar, Alan S. Krober, Peter R. Oeltgen, Jeffrey R. Jones, Steven F. Bolling, Tsung-Ping Su, Dimitra J. Kaftani, David S. Bruce, Noel D. Horton, Evans C. Bailey |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Narcotics Hibernation medicine.medical_specialty Enkephalin Physiology Molecular Sequence Data Alpha (ethology) Biology Biochemistry chemistry.chemical_compound Vas Deferens Receptors Opioid delta Internal medicine medicine Animals Amino Acid Sequence RNA Messenger Receptor Molecular Biology Glycoproteins Base Sequence Albumin Biological activity Blood Proteins Enkephalin Leucine-2-Alanine Endocrinology Liver chemistry Opioid Marmota Electrophoresis Polyacrylamide Gel DADLE Sequence Alignment Sequence Analysis Protein Binding medicine.drug |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 119:787-805 |
| ISSN: | 1096-4959 |
| DOI: | 10.1016/s0305-0491(98)00056-x |
| Popis: | Previous studies show that infusion of hibernating woodchuck albumin (HWA) induces hibernation in summer-active ground squirrels and results in profound behavioral and physiological depression in primates. These effects are reversed by the administration of opiate antagonists, suggesting that the putative hibernation induction trigger (HIT) may act through opioid receptors. We have demonstrated that both HIT-containing plasma and the synthetic alpha opioid D-Ala2-D-Leu5-enkephalin (DADLE), which mimics the activity of HIT in hibernators, extend tissue survival time of a multi-organ autoperfusion system by 3-fold. In this study we present the first data showing biological activity with a much more highly purified plasma fraction from hibernating woodchucks, identified as the hibernation-related factor (HRF). Both the HRF and DADLE show opiate-like contractile inhibition in the mouse vas deferens (Mvd) bioassay. We also have preliminary evidence in an isolated rabbit heart preparation indicating that the HRF and DADLE act similarly to restore left ventricular function following global myocardial ischemia. Furthermore, we have partially sequenced an alpha 1-glycoprotein-like 88 kDa hibernation-related protein (p88 HRP) present in this fraction, which may prove to be the blood-borne HIT molecule. |
| Databáze: | OpenAIRE |
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