The adult human subventricular zone

Autor: Eleonora Aronica, Sophia F A M de Sonnaville, Tasmin Deering, Dick F. Swaab, Lidia De Filippis, Annemiek van Berkel, Rainer Glass, Vanessa Donega, Elly M. Hol, Martina Moeton, Pierre Robe, Jinte Middeldorp, Wilma D.J. van de Berg, Jacqueline A. Sluijs, Angelo Luigi Vescovi, Simone A. van den Berge, Miriam E van Strien
Přispěvatelé: Netherlands Institute for Neuroscience (NIN), Anatomy and neurosciences, de Sonnaville, S, van Strien, M, Middeldorp, J, Sluijs, J, van den Berge, S, Moeton, M, Donega, V, van Berkel, A, Deering, T, De Filippis, L, Vescovi, A, Aronica, E, Glass, R, van de Berg, W, Swaab, D, Robe, P, Hol, E
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Brain communications, 2(2)
Brain Communications
Brain Communications, 2(2)
de Sonnaville, S F A M, van Strien, M E, Middeldorp, J, Sluijs, J A, van den Berge, S A, Moeton, M, Donega, V, van Berkel, A, Deering, T, De Filippis, L, Vescovi, A L, Aronica, E, Glass, R, van de Berg, W D J, Swaab, D F, Robe, P A & Hol, E M 2020, ' The adult human subventricular zone : partial ependymal coverage and proliferative capacity of cerebrospinal fluid ', Brain Communications, vol. 2, no. 2, pp. fcaa150 . https://doi.org/10.1093/braincomms/fcaa150
ISSN: 2632-1297
Popis: Neurogenesis continues throughout adulthood in specialized regions of the brain. One of these regions is the subventricular zone. During brain development, neurogenesis is regulated by a complex interplay of intrinsic and extrinsic cues that control stem-cell survival, renewal and cell lineage specification. Cerebrospinal fluid (CSF) is an integral part of the neurogenic niche in development as it is in direct contact with radial glial cells, and it is important in regulating proliferation and migration. Yet, the effect of CSF on neural stem cells in the subventricular zone of the adult human brain is unknown. We hypothesized a persistent stimulating effect of ventricular CSF on neural stem cells in adulthood, based on the literature, describing bulging accumulations of subventricular cells where CSF is in direct contact with the subventricular zone. Here, we show by immunohistochemistry on post-mortem adult human subventricular zone sections that neural stem cells are in close contact with CSF via protrusions through both intact and incomplete ependymal layers. We are the first to systematically quantify subventricular glial nodules denuded of ependyma and consisting of proliferating neural stem and progenitor cells, and showed that they are present from foetal age until adulthood. Neurosphere, cell motility and differentiation assays as well as analyses of RNA expression were used to assess the effects of CSF of adult humans on primary neural stem cells and a human immortalized neural stem cell line. We show that human ventricular CSF increases proliferation and decreases motility of neural stem cells. Our results also indicate that adult CSF pushes neural stem cells from a relative quiescent to a more active state and promotes neuronal over astrocytic lineage differentiation. Thus, CSF continues to stimulate neural stem cells throughout aging.
De Sonnaville et al. demonstrate that subventricular glial nodules in the human subventricular zone—denuded of ependyma—consist of accumulating proliferating neural stem and progenitor cells, corroborating in vitro findings that adult cerebrospinal fluid retains the capacity to stimulate proliferation, influence motility and promote differentiation of neural stem cells.
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Databáze: OpenAIRE
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