Human osteoarthritic chondrocytes exposed to extremely low-frequency electromagnetic fields (ELF) and therapeutic application of musically modulated electromagnetic fields (TAMMEF) systems: a comparative study
| Autor: | Ranuccio Nuti, Nila Volpi, Nicola Giordano, Roberto Leoncini, Antonella Fioravanti, A Montella, Claudio Corallo, Emilio Battisti, Massimo Guarna, Daniela Vannoni, A. Albanese, G. Landi, Daniela Franci |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Alginate beads cultures Cell Survival Immunology Osteoarthritis Matrix (biology) Cell morphology Chondrocyte TAMMEF Chondrocytes Electromagnetic Fields Rheumatology Microscopy Electron Transmission In vivo Electromagnetic fields Human primary chondrocytes Microscopy Immunology and Allergy Medicine Humans Viability assay Cells Cultured Aged Aged 80 and over business.industry Cartilage Anatomy Middle Aged medicine.disease medicine.anatomical_structure Biophysics Female business Music |
| Zdroj: | Rheumatology international. 33(6) |
| ISSN: | 1437-160X |
| Popis: | Osteoarthritis (OA) is the most common joint disease, characterized by matrix degradation and changes in chondrocyte morphology and metabolism. Literature reported that electromagnetic fields (EMFs) can produce benefits in OA patients, even if EMFs mechanism of action is debated. Human osteoarthritic chondrocytes isolated from femoral heads were cultured in vitro in bidimensional (2-D) flasks and in three-dimensional (3-D) alginate beads to mimic closely cartilage environment in vivo. Cells were exposed 30 min/day for 2 weeks to extremely low-frequency electromagnetic field (ELF) with fixed frequency (100 Hz) and to therapeutic application of musically modulated electromagnetic field (TAMMEF) with variable frequencies, intensities, and waveforms. Cell viability was measured at days 7 and 14, while healthy-cell density, heavily vacuolized (hv) cell density, and cluster density were measured by light microscopy only for 3-D cultures after treatments. Cell morphology was observed for 2-D and 3-D cultures by transmission electron microscopy (TEM). Chondrocyte exposure to TAMMEF enhances cell viability at days 7 and 14 compared to ELF. Light microscopy analysis showed that TAMMEF enhances healthy-cell density, reduces hv-cell density and clustering, compared to ELF. Furthermore, TEM analysis showed different morphology for 2-D (fibroblast-like) and 3-D (rounded shape) cultures, confirming light microscopy results. In conclusion, EMFs are effective and safe for OA chondrocytes. TAMMEF can positively interfere with OA chondrocytes representing an innovative non-pharmacological approach to treat OA. |
| Databáze: | OpenAIRE |
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