Expression of epithelial neutrophil-activating peptide 78 in cultured human endometrial stromal cells
| Autor: | Kengo Kai, Kayo Fujisawa, Kaei Nasu, Kazuyo Arima, Isao Miyakawa, Masakazu Nishida |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Lipopolysaccharides
Chemokine CXCL5 Embryology medicine.medical_specialty Chemokine Stromal cell Lipopolysaccharide Biology Endometrium chemistry.chemical_compound Stroma In vivo Internal medicine Follicular phase Genetics medicine Humans Molecular Biology Cells Cultured reproductive and urinary physiology Tumor Necrosis Factor-alpha urogenital system Interleukin-8 Obstetrics and Gynecology Cell Biology Culture Media Cell biology medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry biology.protein Immunohistochemistry Female Stromal Cells Chemokines CXC Interleukin-1 Developmental Biology |
| Zdroj: | Molecular Human Reproduction. 7:453-458 |
| ISSN: | 1460-2407 1360-9947 |
| Popis: | It has been demonstrated that human endometrial stromal cells (ESC) produce a variety of chemokines in vivo and in vitro. To evaluate the expression of epithelial neutrophil-activating peptide 78 (ENA-78) in the endometrium, concentrations of ENA-78 in cyclic endometrial tissues were measured using enzyme-linked immunosorbent assay. The expression of ENA-78 was also detected in cyclic endometrium by immunohistochemistry. Endometrial tissues in the secretory phase contained higher amounts of ENA-78 protein than did those in the proliferative phase. Immunofluorescence staining revealed that ENA-78 protein was localized mainly in the stroma of endometrium. In addition, to evaluate the involvement of inflammatory mediators and ovarian steroid hormones in the production of ENA-78 by ESC was evaluated by in-vitro studies. Unstimulated ESC constitutively secreted ENA-78. Progesterone, lipopolysaccharide, tumour necrosis factor-alpha, and interleukin-1beta significantly stimulated the expression of ENA-78 by ESC. It is suggested that the production of ENA-78 by ESC is regulated by progesterone as well as by the inflammatory mediators. The modulation of ENA-78 concentration in the local environment by these mediators may contribute to the normal and pathological processes of human reproduction through regulation of leukocyte trafficking into the endometrium. |
| Databáze: | OpenAIRE |
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