Optimizing the compatibility of paired-herb in an ancient Chinese herbal decoction Kai-Xin-San in activating neurofilament expression in cultured PC12 cells
| Autor: | Huangquan Lin, Ning Li, KY Zhu, Gui-Zhong Xin, Karl Wah Keung Tsim, Sherry L. Xu, Maitinuer Maiwulanjiang, Kelly Y.C. Lam, Lu Yan, Tina T. X. Dong |
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| Rok vydání: | 2015 |
| Předmět: |
Pharmacology
food.ingredient Neurofilament Traditional medicine biology business.industry Intermediate Filaments Decoction Promoter biology.organism_classification PC12 Cells complex mixtures Rats Rhizome Ginseng food Gene Expression Regulation Promoter activity Herb Drug Discovery Polygala tenuifolia Animals Medicine business Drugs Chinese Herbal |
| Zdroj: | Journal of Ethnopharmacology. 162:155-162 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2014.12.049 |
| Popis: | Ethnopharmacological relevance Kai-Xin-San (KXS), a well-known traditional Chinese herbal decoction, has been widely used to treat mental depression and memory loss in China. It has a combination of four herbs: Ginseng Radix et Rhizoma (GR; root and rhizome of Panax ginseng C. A. Mey.), Polygalae Radix (PR; root of Polygala tenuifolia Wild.), Acori Tatarinowii Rhizoma (ATR; rhizome of Acorus tatarinowii Schott), and Poria (PO; sclerotium of Poriacocos (Schw.) Wolf), from which a pairing of two herbs was considered as paired-herb, such as the pairing of GR–PR and ATR–PO. The depression-induced neural cell loss is one of the major pathogenesis in depression. Here, an optimized KXS by changing the ratio of paired-herbs in KXS was demonstrated aiming at promoting neural cell differentiation. Materials and methods Quantitative assessment of chemical markers in each herbal extract was determined by LC–MS. Promoters of neurofilaments, NF68 and NF200, linked with luciferase reporter gene (pNF68-Luc and pNF200-Luc) were applied in cultured pheochromocytoma (PC12) cells to study the transcriptional activation of each herbal extract. The effect of GR–PR and ATR–PO in improving NF promoter activity was analyzed by Compusyn software. The activation of PKA was indicated. Results In PC12 cells, an optimized KXS named KXS1:5 having 1:5 of GR–PR:ATR–PO had greater capability in promoting the expression of neurofilament. The synergistic effect of GR–PR and ATR–PO on the improved efficiency was further determined. Moreover, the treatment of H89, a PKA inhibitor, significantly inhibited the induced NF promoter activity. Conclusion These results indicated an optimized KXS by optimizing the compatibility of paired-herb and this compatibility was proven to exert synergistic effect. Moreover, the underlying mechanism was mediated by a PKA signaling pathway. |
| Databáze: | OpenAIRE |
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