Variable germline and embryonic instability of the human minisatellite MS32 (D1S8) in transgenic mice
| Autor: | Maxine J. Allen, Alec J. Jeffreys, S.C. Barton, M. A. Surani, Philippe Bois, M.L. Norris, Andrew Collick |
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| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Male
Transgene Minisatellite Repeat Molecular Sequence Data Gene Dosage Mitosis Locus (genetics) Mice Transgenic Biology DNA Satellite General Biochemistry Genetics and Molecular Biology Germline Mice Germline mutation Tandem repeat Animals Humans Repeated sequence Molecular Biology Germ-Line Mutation Sequence Deletion Genetics General Immunology and Microbiology Base Sequence General Neuroscience Embryo Mammalian Molecular biology Pedigree Mice Inbred C57BL Meiosis Minisatellite Mice Inbred CBA Female Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | Tandem repeat loci such as minisatellites and trinucleotide repeats frequently show instability. We have investigated mutation at human minisatellite MS32 (locus D1S8) transferred to transgenic mice. Three lines of hemizygous transgenic mice were studied. A single-copy line (110D) was seen to be relatively stable, whilst two multicopy lines showed structural instability of the transgene in pedigrees (lines 109 and 110A). For both these lines, mutant structures were detected as a result of mutation events having occurred in the germline or early embryo. Structural changes seen included gain or loss of minisatellite repeat units (110A and 109), alteration of DNA flanking the minisatellite repeat array (109 only) or deletion of the entire transgene (109 only). This work demonstrates that tandem repeat transgenes can show instability and thus provide additional systems for the analysis of repetitive DNA structural change in mice. |
| Databáze: | OpenAIRE |
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