Reactive oxygen species, glutathione, and vitamin E concentrations in dogs with hemolytic or nonhemolytic anemia

Autor: Andrea Pires dos Santos, Andrew D Woolcock, John A. Christian, George E. Moore, Priscila B S Serpa
Rok vydání: 2020
Předmět:
Hemolytic anemia
Anemia
Hemolytic
medicine.medical_specialty
Erythrocytes
040301 veterinary sciences
Anemia
medicine.medical_treatment
Standard Article
030204 cardiovascular system & hematology
medicine.disease_cause
Flow cytometry
0403 veterinary science
03 medical and health sciences
chemistry.chemical_compound
Dogs
0302 clinical medicine
Internal medicine
medicine
Animals
Vitamin E
Dog Diseases
Prospective Studies
free radical
chemistry.chemical_classification
Reactive oxygen species
General Veterinary
medicine.diagnostic_test
business.industry
Hematology
04 agricultural and veterinary sciences
Glutathione
medicine.disease
Standard Articles
Hemolysis
Oxidative Stress
antioxidants
Cross-Sectional Studies
Endocrinology
chemistry
SMALL ANIMAL
hemolysis
Reactive Oxygen Species
business
Oxidative stress
Zdroj: Journal of Veterinary Internal Medicine
ISSN: 1939-1676
0891-6640
DOI: 10.1111/jvim.15926
Popis: Background Red blood cells (RBC) are uniquely susceptible to oxidative injury. Oxidative stress is both a cause for, and effect, of anemia in people but this has been minimally documented in dogs. Objective To describe direct and indirect markers of oxidative stress in anemic dogs. Hypothesis Anemic dogs will have oxidative stress when compared to healthy dogs. Animals Forty‐seven dogs with anemia (10 with hemolytic anemia) and 70 healthy control dogs. Methods Prospective, cross‐sectional study. Anemic dogs were identified from the patient population, and medical records were reviewed to classify the anemia as hemolytic or nonhemolytic. Flow cytometry was used to detect reactive oxygen species (ROS) in erythrocyte isolates. Reduced glutathione (GSH) concentrations were measured in both plasma and hemolysate samples, and vitamin E was measured in serum. Results Anemic dogs (both hemolytic and nonhemolytic) had significantly lower median RBC hemolysate GSH concentrations (3.1 μM [0.4‐30.8]) when compared to healthy dogs (7.0 μM [0.5‐29.7]; P = .03). Dogs with hemolytic anemia had significantly higher median plasma GSH (7.6 μM [0.4‐17.8]) when compared to dogs with nonhemolytic anemia (1.6 μM [0.01‐7.1]; P = .04) and healthy dogs (2.8 μM [0.1‐29.9]; P
Databáze: OpenAIRE
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