Increased APN/CD13 and acid aminopeptidase activities in head and neck squamous cell carcinoma

Autor: Francisco Santaolalla, Agustín Martínez Ibargüen, Aitor Zabala, Javier Gil, Adolfo Varona, Itxaro Perez, Gorka Larrinaga, Jon Irazusta, Lorena Blanco
Rok vydání: 2009
Předmět:
Zdroj: Headneck. 31(10)
ISSN: 1097-0347
Popis: Background. Involvement of peptidases in carci- nogenic processes of several tumor types has been investigated in recent years. Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers worldwide and accounts for more than 90% of all head and neck cancers. Increased understanding of its pathophysiology has led to impli- cation of several proteinases, specially matrix metalloprotei- nases, in its genesis, growth, and dissemination. However, very little is known about involvement of peptidases in this neoplasm. Methods. Seventeen HNSCC tissue samples were selected for the study. Tumor and normal tissue samples were frozen for enzymatic study. The catalytic activity for a pool of pepti- dases (PSA, APN/CD13, APB, APA, Asp-AP, CAP, DPPIV/ CD26, NEP/CD10, and PGI) was measured fluorometrically. Results. The activity of 2 cell surface aminopeptidases (APN/CD13 and APA) and a cytosolic aminopeptidase (Asp- AP) was significantly increased in HNSCC tissues. Conclusions. These data show the involvement of cell sur- face and cytosolic peptidases in the mechanisms underlying HNSCC. V C 2009 Wiley Periodicals, Inc. Head Neck 31: 1335-1340, 2009
Databáze: OpenAIRE
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