Identification of CG-1, a natural peptide antibiotic derived from human neutrophil cathepsin G
| Autor: | Xiao-Dan Qu, K. T. Miyasaki, Robert I. Lehrer, S. S. L. Harwig, Yoon Cho |
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| Rok vydání: | 1995 |
| Předmět: |
0301 basic medicine
Cathepsin G Neutrophils Molecular Sequence Data Peptide Cytoplasmic Granules Cathepsin C 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cathepsin O Bacterial Proteins Cathepsin L1 Humans Prodrugs Amino Acid Sequence Peptide sequence Cathepsin chemistry.chemical_classification Serine Endopeptidases Membrane Proteins 030206 dentistry General Medicine Blood Proteins Cathepsins Anti-Bacterial Agents Granzyme B 030104 developmental biology chemistry Biochemistry Peptides |
| Zdroj: | Advances in dental research. 9(1) |
| ISSN: | 0895-9374 |
| Popis: | Cathepsin G is a neutral serine protease of the granzyme B family which is found in human PMN, cells known to be important in the defense of the periodontium against periodontal bacteria. We propose that cathepsin G serves as a "pro-antibiotic" containing peptide domains which express selective antibiotic properties. In this study, we used HPLC to separate the low-molecular-weight peptides derived from the ultrafiltrate of a granule extract from unstimulated PMN. One of the peptides exhibited intense bactericidal activity as determined by radial diffusion overlay assay (against Escherichia coli ML-35P), an amino-terminal sequence "RVSSFLPWIR...", and a 3.1-kDa molecular mass determined by electrospray ionization-mass spectrometry. The sequence and mass are consistent with the C-terminus of cathepsin G deduced by cDNA analysis. These findings support the hypothesis that antibiotic peptides derived from cathepsin G occur naturally in human PMN. Since this is the first naturally occurring antibiotic peptide derived from cathepsin G, we designate it "CG-1". |
| Databáze: | OpenAIRE |
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