Hybrid donor-acceptor polymer nanoparticles and combination antibiotic for mitigation of pathogenic bacteria and biofilms
| Autor: | Narayanan Kuthirummal, Santu Sarkar, Shaina A. Yates-Alston, Nicole H. Levi, Matthew Cochran |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Fluorescence-lifetime imaging microscopy Polymers Streptococcus pyogenes medicine.drug_class Antibiotics Nanoparticle Microbial Sensitivity Tests Polysaccharide medicine.disease_cause Microbiology Elastic Modulus Streptococcal Infections medicine Humans Hyperthermia Molecular Biology chemistry.chemical_classification Microbial Viability Clindamycin Biofilm Pathogenic bacteria Polymer Staphylococcal Infections Anti-Bacterial Agents chemistry Biofilms Nanoparticles medicine.drug |
| Zdroj: | Journal of Microbiological Methods. 190:106328 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2021.106328 |
| Popis: | Biofilms pose a significant clinical problem in skin and soft tissue infections. Their resistance to antibiotics has spurred investigations into alternative treatments, such as nanoparticle-mediated photothermal ablation. Non-toxic Hybrid Donor- Acceptor (DA) Polymer nanoParticles (H-DAPPs) were developed for fluorescence imaging (using poly(3-hexylthiophene-2,5 diyl) (P3HT)) and rapid, near-infrared photothermal ablation (NIR- PTA) (using poly[4,4-bis(2-ethylhexyl)-cyclopenta[2,1-b;3,4-b’]dithiophene-2,6-diyl-alt−2,1,3-benzoselenadiazole-4,7-diyl] (PCPDTBSe)). H-DAPPs were evaluated alone, and in combination with antibiotics, against planktonic S. aureus and S. pyogenes , and S. aureus biofilms. H-DAPPs NIR-PTA (15–700 μg/ mL) can generate rapid temperature changes of 27.6–73.1 °C, which can eradicate planktonic bacterial populations and reduce biofilm bacterial viability by more than 4- log (> 99.99%) with exposure to 60 s of 800 nm light. Reductions were confirmed via confocal analysis, which suggested that H-DAPPs PTA caused bacterial inactivation within the biofilms, but did not significantly reduce biofilm polysaccharides. SEM imaging revealed structural changes in biofilms after H-DAPPs PTA. S. aureus biofilms challenged with 100 μg/mL of H-DAPPs (H-DAPPs-100) to induce an average temperature of 55.1 °C, and the minimum biofilm eradication concentration (MBEC) of clindamycin, resulted in up to ~3- log decrease in bacterial viability compared to untreated biofilms and those administered H-DAPPs-100 PTA only, and up to ~2- log compared to biofilms administered only clindamycin. This study demonstrates that polymer nanoparticle PTA can mitigate biofilm infection and may improve antimicrobial efficacy. |
| Databáze: | OpenAIRE |
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