Ptk7-Deficient Mice Have Decreased Hematopoietic Stem Cell Pools as a Result of Deregulated Proliferation and Migration

Autor: Marilyn Giordano, Florence Bardin, Jean-Christophe Orsoni, Sylvie Marchetto, Anne-Catherine Lhoumeau, Frédérique Lembo, Marie-Laure Arcangeli, Michel Aurrand-Lions, Jean-Paul Borg, Maria De Grandis
Přispěvatelé: Centre de Recherche en Cancérologie de Marseille (CRCM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), This work was supported by Institut National du Cancer (INCa) (Projet Libre INCa2012-108), the Site de Recherche Inte´gre´e en Cance´rologie program (INCa-Direction ge´ne´rale de l’offre de soins-INSERM 6038), and Canceropole Provence-Alpes-Coˆte d’Azur (all to M.A.-L. and J.-P.B.). A.-C.L. and M.-L.A. were supportedby la Fondation pour la Recherche Me´dicale. M.D.G. was supported by la Fondationde France. J.-P.B.’s laboratory is supported by INSERM, Institut Paoli-Calmettes,and La Ligue Nationale Contre le Cancer ('Equipe labellise´e'). J.-P.B. is a scholar atthe Institut Universitaire de France., Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), BORG, Jean-Paul
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
[SDV.MHEP.HEM] Life Sciences [q-bio]/Human health and pathology/Hematology
Immunology
Stem cell factor
[SDV.CAN]Life Sciences [q-bio]/Cancer
Biology
[SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Cell Line
03 medical and health sciences
Mice
[SDV.CAN] Life Sciences [q-bio]/Cancer
Cell Movement
stem cells
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
medicine
Cell Adhesion
Immunology and Allergy
Animals
Humans
Progenitor cell
Cell Proliferation
Mice
Knockout
Wnt signaling pathway
Hematopoietic stem cell
Receptor Protein-Tyrosine Kinases
[SDV.BDD.EO] Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis
[SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology
Hematopoietic Stem Cells
PTK7
hematopoiesis
Cell biology
Endothelial stem cell
Mice
Inbred C57BL
cell polarity
030104 developmental biology
medicine.anatomical_structure
[SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis
Stem cell
Homing (hematopoietic)
Adult stem cell
Signal Transduction
Zdroj: Journal of Immunology
Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2016, ⟨10.4049/jimmunol.1500680⟩
Journal of Immunology, 2016, ⟨10.4049/jimmunol.1500680⟩
ISSN: 0022-1767
1550-6606
DOI: 10.4049/jimmunol.1500680⟩
Popis: Hematopoietic stem cells (HSCs) located in adult bone marrow or fetal liver in mammals produce all cells from the blood system. At the top of the hierarchy are long-term HSCs endowed with lifelong self-renewal and differentiation properties. These features are controlled through key microenvironmental cues and regulatory pathways, such as Wnt signaling. We showed previously that PTK7, a tyrosine kinase receptor involved in planar cell polarity, plays a role in epithelial Wnt signaling; however, its function in hematopoiesis has remained unexplored. In this article, we show that PTK7 is expressed by hematopoietic stem and progenitor cells, with the highest level of protein expression found on HSCs. Taking advantage of a Ptk7-deficient mouse strain, we demonstrate that loss of Ptk7 leads to a diminished pool of HSCs but does not affect in vitro or in vivo hematopoietic cell differentiation. This is correlated with increased quiescence and reduced homing abilities of Ptk7-deficient hematopoietic stem and progenitor cells, unraveling novel and unexpected functions for planar cell polarity pathways in HSC fate.
Databáze: OpenAIRE
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