Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside
| Autor: | Xiao-kun Wei, Changlu Wang, Qingbao Ding, Lin Ou, Yong-li Guo, Lu Zhang |
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| Rok vydání: | 2008 |
| Předmět: |
Cytidine monophosphate
Biotechnology and Biochemistry General Veterinary biology Purine nucleoside phosphorylase Cytidine Enterobacter aerogenes General Medicine biology.organism_classification General Biochemistry Genetics and Molecular Biology carbohydrates (lipids) chemistry.chemical_compound Biochemistry chemistry Enzyme Induction Uridine phosphorylase Cytidine Monophosphate Pentosyltransferases General Pharmacology Toxicology and Pharmaceutics Thymidine phosphorylase Nucleoside Nucleotide salvage Vidarabine |
| Zdroj: | Journal of Zhejiang University SCIENCE B. 9:520-526 |
| ISSN: | 1862-1783 1673-1581 |
| Popis: | Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5'-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced. |
| Databáze: | OpenAIRE |
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