Development of Macrophages with Altered Actin Organization in the Absence of MafB
| Autor: | Sandrine Sarrazin, Peer Mohideen, Noushine Mossadegh, Athar Aziz, Laurent Vanhille, Claas Otto, Youssef Bakri, Michael H. Sieweke, Louise M. Kelly |
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| Přispěvatelé: | Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Spleen
Cellular differentiation MESH: Flow Cytometry MESH: Animals Newborn Mice 0302 clinical medicine Macrophage MESH: Animals 0303 health sciences MafB Transcription Factor Cell Differentiation Articles MESH: MafB Transcription Factor Flow Cytometry MESH: Gene Expression Regulation Haematopoiesis medicine.anatomical_structure Liver MAFB 030220 oncology & carcinogenesis Proto-Oncogene Proteins c-maf [SDV.IMM]Life Sciences [q-bio]/Immunology MESH: Proto-Oncogene Proteins c-maf MESH: Whole-Body Irradiation Whole-Body Irradiation MESH: Cell Differentiation Phagocytosis Hematopoietic System Spleen Biology MESH: Actins 03 medical and health sciences MESH: Gene Expression Profiling Fetus MESH: Mice Inbred C57BL medicine Animals MESH: Hematopoietic System RNA Messenger Transcription Factor MafB Molecular Biology MESH: Mice 030304 developmental biology MESH: RNA Messenger Gene Expression Profiling Macrophages MESH: Embryo MESH: Macrophages MESH: Fetus Cell Biology Embryo Mammalian Molecular biology Actins Mice Inbred C57BL Animals Newborn Gene Expression Regulation MESH: Liver |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 2006, 26 (18), pp.6808-18. ⟨10.1128/MCB.00245-06⟩ Molecular and Cellular Biology, 2006, 26 (18), pp.6808-18. ⟨10.1128/MCB.00245-06⟩ |
| ISSN: | 0270-7306 1098-5549 |
| DOI: | 10.1128/MCB.00245-06⟩ |
| Popis: | In the hematopoietic system the bZip transcription factor MafB is selectively expressed at high levels in monocytes and macrophages and promotes macrophage differentiation in myeloid progenitors, whereas a dominant-negative allele can inhibit this process. To analyze the requirement of MafB for macrophage development, we generated MafB-deficient mice and, due to their neonatal lethal phenotype, analyzed macrophage differentiation in vitro, in the embryo, and in reconstituted mice. Surprisingly we observed in vitro differentiation of macrophages from E14.5 fetal liver (FL) cells and E18.5 splenocytes. Furthermore we found normal numbers of F4/80(+)/Mac-1(+) macrophages and monocytes in fetal liver, spleen, and blood as well as in bone marrow, spleen, and peritoneum of adult MafB(-/-) FL reconstituted mice. MafB(-/-) macrophages showed intact basic macrophage functions such as phagocytosis of latex beads or Listeria monocytogenes and nitric oxide production in response to lipopolysaccharide. By contrast, MafB(-/-) macrophages expressed increased levels of multiple genes involved in actin organization. Consistent with this, phalloidin staining revealed an altered morphology involving increased numbers of branched protrusions of MafB(-/-) macrophages in response to macrophage colony-stimulating factor. Together these data point to an unexpected redundancy of MafB function in macrophage differentiation and a previously unknown role in actin-dependent macrophage morphology. |
| Databáze: | OpenAIRE |
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