Correlation between the GP78 Gene Polymorphism and Coronary Atherosclerotic Heart Disease
| Autor: | Cheng-Dong Qiao, Long Wang, Jian Zhou, Zheng Zhang, Yong-Ming Liu, Bo Yang, Hua-Cheng Li |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male lcsh:Diseases of the circulatory (Cardiovascular) system medicine.medical_specialty Heart disease Genotype Blotting Western Blood lipids Locus (genetics) Enzyme-Linked Immunosorbent Assay Coronary Artery Disease 030204 cardiovascular system & hematology Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Humans Gene Retrospective Studies Medicine(all) Polymorphism Genetic business.industry DNA Middle Aged medicine.disease Atherosclerosis Receptors Autocrine Motility Factor 030104 developmental biology Real-time polymerase chain reaction Endocrinology Blood pressure lcsh:RC666-701 Cardiology Female Gene polymorphism Restriction fragment length polymorphism Cardiology and Cardiovascular Medicine business Follow-Up Studies |
| Zdroj: | Hellenic Journal of Cardiology, Vol 59, Iss 1, Pp 8-13 (2018) |
| ISSN: | 2241-5955 |
| Popis: | Objective: To study the correlation between the GP78 gene polymorphism and blood fat, blood glucose, blood pressure and coronary atherosclerotic heart disease. Methods: A total of 72 patients with coronary atherosclerotic heart disease were selected as the observation group, and 68 healthy participants were selected as the control group. The gp78 gene polymorphism of both groups was studied via polymerase chain reaction-restriction fragment length polymorphism (RFLP). At the same time, the multiple expression quantities of the GP78 gene in the tissues of both groups were tested via fluorogenic quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and Western-blotting assay. Furthermore, the blood fat, blood glucose and blood pressure of subjects in both groups were tested. Results: The percentages of the gp78 gene polymorphisms of Arg/Arg, Arg/Gly and Gly/Gly at the 145 locus of the study subjects in the observation group were 12.3%, 43.2% and 44.5%, respectively, while those in the control group were 74.3%, 11.2% and 14.5%, respectively, and there were significant differences between both groups. Based on the test results of the blood fat, blood glucose and blood pressure of the objects in the observation group and control group, significant differences were found between the two groups (P |
| Databáze: | OpenAIRE |
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