Halomonas salinarum sp. nov., a moderately halophilic bacterium isolated from saline soil in Yingkou, China
| Autor: | Lei Wang, Fang-Ling Li, Ya-Lin Yin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial Halomonas Soil salinity biology Chemistry Fatty Acids Nucleic Acid Hybridization General Medicine Sequence Analysis DNA biology.organism_classification Biochemistry Microbiology Halophile Bacterial Typing Techniques Soil RNA Ribosomal 16S Botany Genetics Molecular Biology Bacteria Phospholipids Phylogeny |
| Zdroj: | Archives of microbiology. 204(8) |
| ISSN: | 1432-072X |
| Popis: | Strains of Halomonas, thought to play vital roles in the environment for their versatility, are ubiquitous in hypersaline environments. A Gram-staining-negative, moderately halotolerant, facultatively aerobic, motile bacterium, designated G5-11T, was isolated from saline soil in Yingkou of Liaoning, China. The cells of strain G5-11T grew at 4-35 ℃ (optimum 30 ℃), at pH 6.0-9.0 (optimum 8.0) and in the presense of 3-15 % (w/v) NaCl (optimum 5 %). The strain could be clearly distinguished from the related species of the genus Halomonas by its phylogenetic position and biochemical characteristics. It presented Q-9 as the major respiratory quinone and the dominant cellular fatty acids were summed feature 8 (C18:1 ω7c/ C18:1 ω6c), C16:0 and summed feature 3 (C16:1 ω7c/ C16:1 ω6c). The polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol as the major components. The G+C content of strain G5-11T genome was 61.0 mol%. 16S rRNA analysis showed that strain G5-11T had the highest similarity to Halomonas niordiana LMG 31227T and Halomonas taeanensis DSM 16463T, both reaching 98.3 %, followed by Halomonas pacifica NBRC 102220T with a value of 95.8 %. Based on phenotypic, chemotaxonomic and phylogenetic inferences, strain G5-11T represents a novel species of the genus Halomonas, for which the name Halomonas salinarum sp. nov. is proposed. The type strain of Halomonas salinarum is G5-11T (=CGMCC 1.12051T=LMG 31677T). |
| Databáze: | OpenAIRE |
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