Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time PCR
| Autor: | Sudchit Chungpivat, Viraphong Lulitanond, Pewpan M. Intapan, Wanchai Maleewong, Piyanan Taweethavonsawat, Wej Choochote, Chairat Tantrawatpan, Penchom Janwan, Tongjit Thanchomnang, Worasak Kaewkong, Oranuch Sanpool |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Brugia pahangi Dirofilaria immitis Population mosquito medicine.disease_cause Real-Time Polymerase Chain Reaction Sensitivity and Specificity Brugia malayi High Resolution Melt Dogs high resolution melting real-time PCR parasitic diseases medicine Brugia Animals Humans Transition Temperature Wuchereria bancrofti education education.field_of_study biology RNA Ribosomal 5S Amplicon biology.organism_classification Virology Infectious Diseases Blood Culicidae Parasitology dog Cats Original Article RNA Helminth |
| Zdroj: | The Korean Journal of Parasitology |
| ISSN: | 1738-0006 0023-4001 |
| Popis: | A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors. |
| Databáze: | OpenAIRE |
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