Production, Purification, and Characterization of Bacillibactin Siderophore of Bacillus subtilis and Its Application for Improvement in Plant Growth and Oil Content in Sesame
| Autor: | Munagala S. Reddy, S. Nithyapriya, R. Z. Sayyed, Daniel Joe Dailin, Sundaram Lalitha, Susila Herlambang, Hesham El Enshasy, Ni Luh Suriani |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
plant growth promotion
Siderophore siderophore bacillibactin Geography Planning and Development Hydrogen cyanide TJ807-830 Bacillus subtilis Management Monitoring Policy and Law Bacillibactin TD194-195 Renewable energy sources Agar plate 03 medical and health sciences chemistry.chemical_compound Sesamum indicum GE1-350 Sesamum Food science 030304 developmental biology 0303 health sciences Rhizosphere Environmental effects of industries and plants biology 030306 microbiology Renewable Energy Sustainability and the Environment P solubilization bioinoculant biology.organism_classification Environmental sciences chemistry Bacteria |
| Zdroj: | Sustainability Volume 13 Issue 10 Sustainability, Vol 13, Iss 5394, p 5394 (2021) |
| ISSN: | 2071-1050 |
| Popis: | Siderophores are low molecular weight secondary metabolites produced by microorganisms under low iron stress as a specific iron chelator. In the present study, a rhizospheric bacterium was isolated from the rhizosphere of sesame plants from Salem district, Tamil Nadu, India and later identified as Bacillus subtilis LSBS2. It exhibited multiple plant-growth-promoting (PGP) traits such as hydrogen cyanide (HCN), ammonia, and indole acetic acid (IAA), and solubilized phosphate. The chrome azurol sulphonate (CAS) agar plate assay was used to screen the siderophore production of LSBS2 and quantitatively the isolate produced 296 mg/L of siderophores in succinic acid medium. Further characterization of the siderophore revealed that the isolate produced catecholate siderophore bacillibactin. A pot culture experiment was used to explore the effect of LSBS2 and its siderophore in promoting iron absorption and plant growth of Sesamum indicum L. Data from the present study revealed that the multifarious Bacillus sp. LSBS2 could be exploited as a potential bioinoculant for growth and yield improvement in S. indicum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|