HEMA-induced oxidative stress inhibits NF-κB nuclear translocation and TNF release from LTA- and LPS-stimulated immunocompetent cells
| Autor: | Claudia Waha, Christine Petzel, Wolfgang Buchalla, Helmut Schweikl, Carola Bolay, Marialucia Gallorini, Margaritha Birke, Karl-Anton Hiller |
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| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Materials science Lipopolysaccharide medicine.medical_treatment 02 engineering and technology medicine.disease_cause Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals General Materials Science Secretion General Dentistry Tumor Necrosis Factor-alpha NF-kappa B 030206 dentistry Glutathione 021001 nanoscience & nanotechnology Molecular biology Oxidative Stress RAW 264.7 Cells Cytokine chemistry Mechanics of Materials Methacrylates lipids (amino acids peptides and proteins) Cytokine secretion Tumor necrosis factor alpha Lipoteichoic acid Reactive Oxygen Species 0210 nano-technology Oxidative stress |
| Zdroj: | Dental Materials. 37:175-190 |
| ISSN: | 0109-5641 |
| DOI: | 10.1016/j.dental.2020.10.029 |
| Popis: | Objective: The release of inflammatory cytokines from antigen-stimulated cells of the immune system is inhibited by resin monomers such as 2-hydroxyethyl methacrylate (HEMA). Although the formation of oxidative stress in cells exposed to HEMA is firmly established, the mechanism behind the inhibited cytokine secretion is only partly known. The present investigation presents evidence regarding the role of HEMA-induced oxidative stress in the secretion of the pro-inflammatory cytokine TNFα from cells exposed to the antigens LTA (lipoteichoic acid) or LPS (lipopolysaccharide) of cariogenic microorganisms using BSO (L-buthionine sulfoximine) or NAC (N-acetyl cysteine) to inhibit or stabilize the amounts of the antioxidant glutathione. Method: RAW264.7 mouse macrophages were treated with LTA, LPS or HEMA in the presence of BSO or NAC for 1h or 24h. Secretion of TNFα from cell cultures was analyzed by ELISA, and the formation of reactive oxygen (ROS) or nitrogen species (RNS) was determined by flow cytometry. Protein expression was detected by Western blotting. Results: The release of TNFα in both LTA- and LPS-exposed cells was decreased by HEMA, and this concentration-dependent inhibitory effect was amplified by BSO or NAC. LTA- and LPS-stimulated expression of the redox-sensitive transcription factor NF-αB (p65) in cell nuclei decreased in the presence of HEMA because the translocation of p65 from the cytosol was prevented by oxidative stress specifically increased by the monomer. Conclusions: A disturbance of the cellular redox balance, particularly induced by HEMA, is a crucial factor in the inhibition of LTA- and LPS-stimulated signalling pathways leading to TNFα secretion. |
| Databáze: | OpenAIRE |
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