Serologic Activity of Fatty Acid Dependent Antibodies in Albumin-Free Systems
| Autor: | R. L. Edwards, William L. Bayer, B. L. Hicklin, S. R. Pierce, Malcolm L. Beck |
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| Rok vydání: | 2003 |
| Předmět: |
Agglutination
Immunology Serology Column chromatography Agglutinin Antibody Specificity Isoantibodies Humans Immunology and Allergy Serum Albumin chemistry.chemical_classification biology Fatty Acids fungi Albumin Fatty acid Hematology Immune complex Immunoglobulin M chemistry Biochemistry Immunoglobulin G biology.protein Serum Globulins Caprylates Antibody |
| Zdroj: | Transfusion. 16:434-436 |
| ISSN: | 0041-1132 |
| Popis: | Fatty acid dependent agglutinin (FADA) refers to serum with the special ability to cause agglutination of red blood cells in the presence of certain fatty acids. The agglutinating mechanism is unclear. It has been proposed that the agglutinin reacts with albumin that has been conformationallly altered by sodium caprylate and that the immune complex is passively adsorbed onto red blood cells. This report presents data that contradicts the proposal assigning a specific role to albumin in the agglutinating mechanism. FADA were isolated by column chromatography of resolubilized euglobulin preparations. No evidence of contamination with albumin was obtained in those IgM fractions possessing FADA activity. We propose, as an alternative explanation, that the serologic activity of FADA depends upon the interaction of IgM agglutinins with haptenic fatty acids. |
| Databáze: | OpenAIRE |
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