Assessment of mycobacterial DNA in cells and tissues of mycobacterial and sarcoid lesions
| Autor: | Detlef Kirsten, U. Greinert, Helgo Magnussen, J Gerdes, J. Galle, H D Flad, M. Duchrow, E. Richter, S Rüsch-Gerdes, Max Schlaak, C Schlüter |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Pulmonary and Respiratory Medicine Systemic disease Tuberculosis Sarcoidosis Molecular Sequence Data Mycobacterium Infections Nontuberculous Critical Care and Intensive Care Medicine Polymerase Chain Reaction Sensitivity and Specificity Fluorescence Mycobacterium law.invention Microbiology Mycobacterium tuberculosis Species Specificity law RNA Ribosomal 16S Bronchoscopy Biopsy medicine Humans Lung Tuberculosis Pulmonary Polymerase chain reaction Electrophoresis Agar Gel Mycobacterium Infections Base Sequence medicine.diagnostic_test biology business.industry Nontuberculous Mycobacteria biology.organism_classification medicine.disease Bronchoalveolar lavage Immunology business Bronchoalveolar Lavage Fluid |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 153:375-380 |
| ISSN: | 1535-4970 1073-449X |
| DOI: | 10.1164/ajrccm.153.1.8542146 |
| Popis: | In this study we applied a polymerase chain reaction (PCR) assay for the detection and species-specific identification of mycobacteria to samples from patients with sarcoidosis and mycobacterial infections and from control patients. The PCR-technique is based on the amplification of mycobacterial DNA coding for 16S rRNA, which is present in all mycobacterial species, and on the additional sequencing of the PCR fragment to determine the species. Mycobacterial DNA could be detected in lung tissues and bronchoalveolar lavage cells from cases of tuberculosis and infections with atypical mycobacteria. On the other hand, mycobacterial DNA was amplified only in lung tissue from one patient with sarcoidosis. Twenty-three samples from patients with sarcoidosis were negative for mycobacterial DNA. From our results we conclude that the granulomatous lesions in sarcoidosis may not be due to mycobacterial infections. |
| Databáze: | OpenAIRE |
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