Pyriproxyfen-treated bed nets reduce reproductive fitness and longevity of pyrethroid-resistant Anopheles gambiae under laboratory and field conditions
Autor: | Wamdaogo M. Guelbeogo, James Maas, Rosemary Susan Lees, Sagnon N’Fale, Steven W. Lindsay, John C. Morgan, Hilary Ranson, Philip J. McCall, Nelson Grisales, Dimitri Wendgida Wangrawa |
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Rok vydání: | 2021 |
Předmět: |
Insecticides
Mosquito Control Pyridines Anopheles gambiae RC955-962 Infectious and parasitic diseases RC109-216 Insecticide Resistance Toxicology chemistry.chemical_compound 0302 clinical medicine Arctic medicine. Tropical medicine Pyrethrins media_common 0303 health sciences qx_4 Pyrethroid Reproduction Longevity Fecundity Olyset Duo® 3. Good health Infectious Diseases Juvenile hormone (JH) Female qx_515 medicine.drug media_common.quotation_subject 030231 tropical medicine Fertility Biology 03 medical and health sciences Anopheles Burkina Faso Insect growth regulator parasitic diseases medicine Animals Insecticide-treated nets (ITNs) Insecticide-Treated Bednets 030304 developmental biology Research wa_240 Pyriproxyfen (PPF) biology.organism_classification chemistry Pyrethroid-resistance Parasitology Genetic Fitness Pyriproxyfen Permethrin |
Zdroj: | Malaria journal, 2021, Vol.20(1), pp.273 [Peer Reviewed Journal] Malaria Journal, Vol 20, Iss 1, Pp 1-17 (2021) Malaria Journal |
ISSN: | 1475-2875 |
Popis: | Background The efficacy of insecticide-treated nets (ITNs) containing the insect growth regulator pyriproxyfen (PPF) and pyrethroid insecticides (PPF-ITNs) is being assessed in clinical trials to determine whether they provide greater protection from malaria than standard pyrethroid-treated ITNs in areas where mosquitoes are resistant to pyrethroids. Understanding the entomological mode of action of this new ITN class will aide interpretation of the results from these trials. Methods Anopheles gambiae sensu lato (s.l.) mosquitoes from a susceptible laboratory strain were exposed to PPF-treated netting 24 h, 6 h, and immediately prior to, or 24 h post blood feeding, and the impact on fecundity, fertility and longevity recorded. Pyrethroid-resistant populations were exposed to nets containing permethrin and PPF (PPF-ITNs) in cone bioassays and daily mortality recorded. Mosquitoes were also collected from inside houses pre- and post-distribution of PPF-ITNs in a clinical trial conduced in Burkina Faso; female An. gambiae s.l. were then assessed for fecundity and fertility. Results PPF exposure reduced the median adult lifespan of insecticide-susceptible mosquitoes by 4 to 5 days in all exposure times (p An. gambiae s.l. females were collected from five villages between 1 and 12 months before distribution of PPF-ITNs. Of these mosquitoes, 75% laid eggs and the remaining 25% appeared to have normal ovaries upon dissection. In contrast, only 8.6% of the 631 blood-fed or gravid An. gambiae s.l. collected post PPF-ITN distribution successfully oviposited; 276 (43.7%) did not oviposit but had apparently normal ovaries upon dissection, and 301 (47.7%) did not oviposit and had abnormal eggs upon dissection. Egg numbers were also significantly lower (average of 138/female prior distribution vs 85 post distribution, p Conclusion Exposure to a mixture of PPF and pyrethroids on netting shortens the lifespan of mosquitoes and reduces reproductive output. Sterilization of vectors lasted at least one year under operational conditions. These findings suggest a longer effective lifespan of PPF-pyrethroid nets than reported previously. |
Databáze: | OpenAIRE |
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