Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture
| Autor: | Francis Sluse, Didier Serteyn, Ginette Deby-Dupont, Nicole Schneider, Jean-Philippe Lejeune, Claire Duyckaerts, Ange Mouithys-Mickalad |
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| Rok vydání: | 2007 |
| Předmět: |
Cartilage
Articular medicine.medical_specialty Alginates Cell Survival Glucose uptake Cell Culture Techniques chemistry.chemical_element Cell Count Cell Separation Biology Oxygen Gas phase Electron Transport Complex IV chemistry.chemical_compound Chondrocytes Oxygen Consumption Glucuronic Acid Internal medicine medicine Animals Horses Lactic Acid Cells Cultured Hexuronic Acids Cell Biology General Medicine Metabolism Oxygenation Microspheres Oxygen tension Glucose Endocrinology Mitochondrial respiratory chain L-Glucose chemistry Biochemistry |
| Zdroj: | Cell Biology International. 31:878-886 |
| ISSN: | 1065-6995 |
| Popis: | We investigated the oxygen (O 2 ) uptake of equine articular chondrocytes to assess their reactions to anoxia/re-oxygenation. They were cultured under 5% or 21% gas phase O 2 and at glucose concentrations of 0, 1.0 or 4.5 g/L in the culture medium ( n = 3). Afterwards, the O 2 consumption rate of the chondrocytes was monitored (oxymetry) before and after an anoxia period of 25 min. The glucose consumption and lactate release were measured at the end of the re-oxygenation period. The chondrocytes showed a minimal O 2 consumption rate, which was hardly changed by anoxia. Independently from the O 2 tension, glucose uptake by the cells was about 30% of the available culture medium glucose, thus higher for cells at 4.5 g/L glucose ( n = 3). Lactate release was also independent from O 2 tension, but lower for cells at 4.5 g/L glucose ( n = 3). Our observations indicated that O 2 consumption by equine chondrocytes was very low despite a functional mitochondrial respiratory chain, and nearly insensitive to anoxia/re-oxygenation. But the chondrocytes metabolism was modified by an excess of O 2 and glucose. |
| Databáze: | OpenAIRE |
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