Pgrmc1 Knockout Impairs Oocyte Maturation in Zebrafish
Autor: | Peter Thomas, Yong Zhu, Xin-Jun Wu |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
subfertility Endocrinology Diabetes and Metabolism lcsh:Diseases of the endocrine glands. Clinical endocrinology 03 medical and health sciences Endocrinology 0302 clinical medicine medicine Epidermal growth factor receptor Receptor PGRMC1 Zebrafish Gene knockout Original Research Paqr7 lcsh:RC648-665 biology Chemistry Membrane progestin receptor alpha Oocyte biology.organism_classification progestin Cell biology mPRα Insulin receptor 030104 developmental biology medicine.anatomical_structure Pgrmc1 oocyte maturation biology.protein 030217 neurology & neurosurgery |
Zdroj: | Frontiers in Endocrinology, Vol 9 (2018) Frontiers in Endocrinology |
ISSN: | 1664-2392 |
Popis: | Recent investigations suggest progestin receptor membrane component 1 (PGRMC1) associates with and transports a wide range of molecules such as heme, cytochromes P450, steroids with 21 carbons, membrane progestin receptor alpha (mPRα/Paqr7), epidermal growth factor receptor (EGFR), and insulin receptor. It is difficult to discriminate the true functions of PGRMC1 from the functions of its associated molecules using biochemical and pharmacological approaches. To determine the physiological function(s) of PGRMC1, we generated global knockouts for pgrmc1 (pgrmc1−/−) in zebrafish. We found a reduction in both spawning frequency and the number of embryos produced by female mutants. We also observed reduced sensitivity of fully-grown immature oocytes to a progestin hormone and a reduced number of oocytes undergone meiotic maturation both in vivo and in vitro in pgrmc1−/−. This reduced sensitivity to progestin corresponds well with significant reduced expression of mPRα, the receptor mainly responsible for mediating oocyte maturation and meiosis resumption in fish. The results provide in vivo and in vitro evidence for the physiological functions of Pgrmc1 in oocyte maturation and fertility, as well as a plausible molecular mechanism via regulation of mPRα, which in turn directly regulates oocyte maturation and affects fertility in zebrafish. |
Databáze: | OpenAIRE |
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