The effect of Zika virus infection in the ferret
| Autor: | Francis T. Djankpa, Bernard J. Dardzinski, Laura D. Reyes, William G. Valiant, Mitali Chatterjee, Carlo Pierpaoli, Sharon L. Juliano, Joseph J. Mattapallil, Elizabeth B. Hutchinson |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microcephaly vasculature neural progenitor Neurotropism Physiology Disease Biology Zika virus neural development 03 medical and health sciences 0302 clinical medicine Immune system medicine Animals RRID:AB_234119 microcephaly Research Articles Zika Virus Infection General Neuroscience diffusion tensor MRI Ferrets RRID:AB_609914 Brain medicine.disease biology.organism_classification RRID:AB_726362 Corticogenesis Disease Models Animal 030104 developmental biology Animals Newborn RRID:AB_11217435 Gestation Immunohistochemistry 030217 neurology & neurosurgery Research Article CT |
| Zdroj: | The Journal of Comparative Neurology |
| ISSN: | 1096-9861 |
| Popis: | Although initial observations of infections with the Zika virus describe a mild illness, more recent reports show that infections by Zika result in neurotropism. In 2015, substantial congenital malformations were observed, with numerous infants born with microcephaly in Brazil. To study the underlying mechanism and effects of the disease, it is critical to find suitable animal models. Rodents lack an immune system parallel to humans and also have lissencephalic brains, which are likely to react differently to infections. As the smallest gyrencephalic mammal, ferrets may provide an important animal model to study the Zika virus, as their brains share many characteristics with humans. To evaluate the prospect of using ferrets to study Zika virus infection, we injected seven pregnant jills with the PR strain subcutaneously on gestational day 21, corresponding to the initiation of corticogenesis. These injections resulted in mixed effects. Two animals died of apparent infection, and all kits were resorbed in another animal that did not die. The other four animals remained pregnant until gestational day 40, when the kits were delivered by caesarian section. We evaluated the animals using CT, MRI, diffusion tensor imaging, and immunohistochemistry. The kits displayed a number of features compatible with an infection that impacted both the brain and skull. The outcomes, however, were variable and differed within and across litters, which ranged from the absence of observable abnormalities to prominent changes, suggesting differential vulnerability of kits to infection by the Zika virus or to subsequent mechanisms of neurodevelopmental disruption. |
| Databáze: | OpenAIRE |
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