| Autor: |
Paul J. Kim, Michael Olymbios, Alfonso Siu, Omar Wever Pinzon, Eric Adler, Nathan Liang, Ryan Swenerton, Jonathan Sternberg, Navchetan Kaur, Ebad Ahmed, Yen-An Chen, Gordon Fehringer, Zachary P. Demko, Paul R. Billings, Josef Stehlik |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation. 41(7) |
| ISSN: |
1557-3117 |
| Popis: |
Endomyocardial biopsy (EMB), the reference surveillance test for acute rejection (AR) in heart transplant (HTx) recipients, is invasive, costly, and shows significant interobserver variability. Recent studies indicate that donor-derived cell-free DNA (dd-cfDNA), obtained non-invasively from blood, is associated with AR and could reduce the frequency of EMB surveillance. The aim of this study was to examine the performance characteristics of a novel test for detecting AR in adult HTx recipients.Plasma samples with contemporaneous EMBs were obtained from HTx recipients. A clinically available SNP-based massively multiplexed-PCR dd-cfDNA assay was used to measure dd-cfDNA fraction. dd-cfDNA fractions were compared with EMB-defined rejection status and test performance was assessed by constructing ROC curves and calculating accuracy measures.A total of 811 samples from 223 patients with dd-cfDNA testing and contemporaneous EMB were eligible for the study. dd-cfDNA fraction was significantly higher in AR (median 0.58%, IQR, 0.13%-1.68%) compared to non-AR (median 0.04%, IQR, 0.01%-0.11%, pThis novel dd-cfDNA test detects AR in HTx recipients with good accuracy and holds promise as a noninvasive test for AR in HTx recipients. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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