Remodeling of the Caenorhabditis elegans non-coding RNA transcriptome by heat shock
| Autor: | William P. Schreiner, Jacob M. Garrigues, Jerry S. Chen, Amy E. Pasquinelli, Delaney C Pagliuso, Antti P. Aalto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Transcriptional Activation
RNA Untranslated RNA Untranslated/genetics Untranslated/genetics Heat-Shock Response/genetics Biology Gene Expression Regulation/genetics Transcriptome Promoter Regions 03 medical and health sciences Caenorhabditis elegans Proteins/genetics 0302 clinical medicine Heat Shock Transcription Factors Genetic Heat shock protein RNA and RNA-protein complexes Genetics Animals Transcriptional Activation/genetics Heat shock Caenorhabditis elegans Caenorhabditis elegans Proteins HSF1 Promoter Regions Genetic Transcription factor 030304 developmental biology 0303 health sciences Promoter Transcriptome/genetics Non-coding RNA biology.organism_classification Cell biology DNA-Binding Proteins Gene Expression Regulation RNA Heat Shock Transcription Factors/chemistry Heat-Shock Response 030217 neurology & neurosurgery Caenorhabditis elegans/genetics DNA-Binding Proteins/genetics |
| Zdroj: | Schreiner, W P, Pagliuso, D C, Garrigues, J M, Chen, J S, Aalto, A P & Pasquinelli, A E 2019, ' Remodeling of the Caenorhabditis elegans non-coding RNA transcriptome by heat shock ', Nucleic Acids Research, vol. 47, no. 18, pp. 9829-9841 . https://doi.org/10.1093/nar/gkz693 Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkz693 |
| Popis: | Elevated temperatures activate a heat shock response (HSR) to protect cells from the pathological effects of protein mis-folding, cellular mis-organization, organelle dysfunction and altered membrane fluidity. This response includes activation of the conserved transcription factor heat shock factor 1 (HSF-1), which binds heat shock elements (HSEs) in the promoters of genes induced by heat shock (HS). The upregulation of protein-coding genes (PCGs), such as heat shock proteins and cytoskeletal regulators, is critical for cellular survival during elevated temperatures. While the transcriptional response of PCGs to HS has been comprehensively analyzed in a variety of organisms, the effect of this stress on the expression of non-coding RNAs (ncRNAs) has not been systematically examined. Here we show that in Caenorhabditis elegans HS induces up- and downregulation of specific ncRNAs from multiple classes, including miRNA, piRNA, lincRNA, pseudogene and repeat elements. Moreover, some ncRNA genes appear to be direct targets of the HSR, as they contain HSF-1 bound HSEs in their promoters and their expression is regulated by this factor during HS. These results demonstrate that multiple ncRNA genes respond to HS, some as direct HSF-1 targets, providing new candidates that may contribute to organismal survival during this stress. |
| Databáze: | OpenAIRE |
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