Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis
| Autor: | Lusheng Fan, Cui Zhang, Bin Gao, Yong Zhang, Ethan Stewart, Jakub Jez, Keiji Nakajima, Xuemei Chen |
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| Rok vydání: | 2022 |
| Předmět: |
cell-to-cell movement
Arabidopsis Genetically Modified Microtubules Medical and Health Sciences Article General Biochemistry Genetics and Molecular Biology miR165/6 Gene Expression Regulation Plant systemic movement Genetics AGO1 loading Molecular Biology microRNA Arabidopsis Proteins Cell Biology Plant Plants Biological Sciences Plants Genetically Modified MicroRNAs non-cell autonomy Gene Expression Regulation Argonaute Proteins Katanin MOR1 microtubule Biotechnology Developmental Biology |
| Zdroj: | Developmental cell, vol 57, iss 8 Dev Cell |
| Popis: | Mobile microRNAs (miRNAs) serve as local and long-distance signals in the developmental patterning and stress responses in plants. However, mechanisms governing the non-cell autonomous activities of miRNAs remain elusive. Here, we show that mutations that disrupt microtubule dynamics are specifically defective for the non-cell autonomous actions of mobile miRNAs, including miR165/6 that is produced in the endodermis and moves to the vasculature to pattern xylem cell fates in Arabidopsis roots. We show that KTN1, a subunit of a microtubule-severing enzyme, is required in source cells to inhibit the loading of miR165/6 into ARGONUATE1 (AGO1), which is cell autonomous, to enable the miRNA to exit the cell. Microtubule disruption enhances the association of miR165/6 with AGO1 in the cytoplasm. These findings suggest that although cell-autonomous miRNAs load onto AGO1 in the nucleus, the cytoplasmic AGO1 loading of mobile miRNAs is a key step regulated by microtubules to promote the range of miRNA cell-to-cell movement. |
| Databáze: | OpenAIRE |
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