Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia
| Autor: | Yongjin Qiu, Michihito Sasaki, Mao Isono, Gabriel Gonzalez, Hirofumi Sawa, Hayato Harima, Yasuko Orba, Ayato Takada, Bernard M. Hang’ombe, Edgar Simulundu, Kosuke Okuya, Eugene C Bwalya, Masahiro Kajihara, Aaron S. Mweene |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Swine
Orthoreovirus Mammalian Reassortment Population Zambia Animals Wild Genome Viral Biology Genome Host Specificity Feces Viral Proteins Chiroptera Virology Prevalence Animals education Mammalian orthoreovirus Phylogeny Recombination Genetic Swine Diseases Genetic diversity education.field_of_study Whole Genome Sequencing business.industry Reoviridae Infections Geographic distribution Livestock business Reassortant Viruses |
| Zdroj: | Journal of General Virology. 101:1027-1036 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/jgv.0.001476 |
| Popis: | Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals. |
| Databáze: | OpenAIRE |
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